PUBLICATION

Lateral line hair cell maturation is a determinant of aminoglycoside susceptibility in zebrafish (Danio rerio)

Authors
Santos, F., Macdonald, G., Rubel, E.W., and Raible, D.W.
ID
ZDB-PUB-060210-13
Date
2006
Source
Hearing Research   213(1-2): 25-33 (Journal)
Registered Authors
Raible, David
Keywords
Ototoxicity, Aminoglycosides, Mechanotransduction, Hearing loss
MeSH Terms
  • Animals
  • Anti-Bacterial Agents/toxicity*
  • Cell Death/drug effects
  • Disease Susceptibility
  • Dose-Response Relationship, Drug
  • Hearing Loss, Sensorineural/chemically induced
  • Larva/cytology
  • Larva/drug effects
  • Larva/growth & development
  • Lateral Line System/cytology
  • Lateral Line System/drug effects*
  • Mechanoreceptors/cytology
  • Mechanoreceptors/drug effects*
  • Mechanoreceptors/growth & development
  • Mechanotransduction, Cellular*/drug effects
  • Neomycin/toxicity*
  • Zebrafish
PubMed
16459035 Full text @ Hear. Res.
Abstract
Developmental differences in hair cell susceptibility to aminoglycoside-induced cell death has been observed in multiple species. Increased sensitivity to aminoglycosides has been temporally correlated with the onset of mechanotransduction-dependent activity. We have used in vivo fluorescent vital dye markers to further investigate the determinants of aminoglycoside induced hair cell death in the lateral line of zebrafish (Danio rerio). Labeling hair cells of the lateral line in vivo with the dyes FM 1-43, To-Pro-3, and Yo-Pro-1 served as reliable indicators of hair cell viability. Results indicate that hair cell maturation is a determinant of developmental differences in susceptibility. The age dependent differences in susceptibility to aminoglycosides are independent of the onset of mechanotransduction-dependent activity as measured by FM 1-43 uptake and independent of hair cell ability to take up fluorescently conjugated aminoglycosides.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping