PUBLICATION
Eya1 is required for lineage-specific differentiation, but not for cell survival in the zebrafish adenohypophysis
- Authors
- Nica, G., Herzog, W., Sonntag, C., Nowak, M., Schwarz, H., Zapata, A.G., Hammerschmidt, M.
- ID
- ZDB-PUB-060210-12
- Date
- 2006
- Source
- Developmental Biology 292(1): 189-204 (Journal)
- Registered Authors
- Hammerschmidt, Matthias, Herzog, Wiebke, Nica, Gabriela, Nowak, Matthias, Sonntag, Carmen
- Keywords
- eya1, six1, Adenohypophysis, aal, dog, Zebrafish, Cell differentiation, Apoptosis
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Apoptosis/genetics
- Apoptosis/physiology
- Cell Survival/genetics
- Cell Survival/physiology
- Mutation
- Cell Differentiation/genetics
- Cell Differentiation/physiology*
- Thyrotropin/biosynthesis
- Thyrotropin/genetics
- Protein Tyrosine Phosphatases/genetics
- Protein Tyrosine Phosphatases/physiology*
- Homeodomain Proteins/biosynthesis
- Homeodomain Proteins/genetics
- Homeodomain Proteins/physiology
- Growth Hormone/biosynthesis
- Growth Hormone/genetics
- Gene Expression Regulation, Developmental/physiology
- Intracellular Signaling Peptides and Proteins/genetics
- Intracellular Signaling Peptides and Proteins/physiology*
- Molecular Sequence Data
- Nerve Tissue Proteins/biosynthesis
- Nerve Tissue Proteins/genetics
- Zebrafish Proteins/biosynthesis
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- LIM-Homeodomain Proteins
- Nuclear Proteins/genetics
- Nuclear Proteins/physiology*
- Transcription Factor Pit-1/biosynthesis
- Transcription Factor Pit-1/genetics
- Pituitary Gland, Anterior/cytology*
- Pituitary Gland, Anterior/embryology*
- Pro-Opiomelanocortin/biosynthesis
- Pro-Opiomelanocortin/genetics
- Cell Lineage/genetics
- Cell Lineage/physiology*
- Zebrafish/embryology*
- Zebrafish/genetics
- Transcription Factors/biosynthesis
- Transcription Factors/genetics
- PubMed
- 16458879 Full text @ Dev. Biol.
Citation
Nica, G., Herzog, W., Sonntag, C., Nowak, M., Schwarz, H., Zapata, A.G., Hammerschmidt, M. (2006) Eya1 is required for lineage-specific differentiation, but not for cell survival in the zebrafish adenohypophysis. Developmental Biology. 292(1):189-204.
Abstract
The homeodomain transcription factor Six1 and its modulator, the protein phosphatase Eya1, cooperate to promote cell differentiation and survival during mouse organ development. Here, we studied the effects caused by loss of eya1 and six1 function on pituitary development in zebrafish. eya1 and six1 are co-expressed in all adenohypophyseal cells. Nevertheless, eya1 (aal, dog) mutants show lineage-specific defects, defining corticotropes, melanotropes, and gonadotropes as an Eya1-dependent lineage, which is complementary to the Pit1 lineage. Furthermore, eya1 is required for maintenance of pit1 expression, leading to subsequent loss of cognate hormone gene expression in thyrotropes and somatotropes of mutant embryos, whereas prolactin expression in lactotropes persists. In contrast to other organs, adenohypophyseal cells of eya1 mutants do not become apoptotic, and the adenohypophysis remains at rather normal size. Also, cells do not trans-differentiate, as in the case of pit1 mutants, but display morphological features characteristic for nonsecretory cells. Some of the adenohypophyseal defects of eya1 mutants are moderately enhanced in combination with antisense-mediated loss of Six1 function, which per se does not affect pituitary cell differentiation. In conclusion, this is the first report of an essential role of Eya1 during pituitary development in vertebrates. Eya1 is required for lineage-specific differentiation of adenohypophyseal cells, but not for their survival, thereby uncoupling the differentiation-promoting and anti-apoptotic effects of Eya proteins seen in other tissues.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping