PUBLICATION

Eya1 is required for lineage-specific differentiation, but not for cell survival in the zebrafish adenohypophysis

Authors
Nica, G., Herzog, W., Sonntag, C., Nowak, M., Schwarz, H., Zapata, A.G., Hammerschmidt, M.
ID
ZDB-PUB-060210-12
Date
2006
Source
Developmental Biology   292(1): 189-204 (Journal)
Registered Authors
Hammerschmidt, Matthias, Herzog, Wiebke, Nica, Gabriela, Nowak, Matthias, Sonntag, Carmen
Keywords
eya1, six1, Adenohypophysis, aal, dog, Zebrafish, Cell differentiation, Apoptosis
MeSH Terms
  • Cell Survival/genetics
  • Cell Survival/physiology
  • Transcription Factors/biosynthesis
  • Transcription Factors/genetics
  • Nerve Tissue Proteins/biosynthesis
  • Nerve Tissue Proteins/genetics
  • Pro-Opiomelanocortin/biosynthesis
  • Pro-Opiomelanocortin/genetics
  • Growth Hormone/biosynthesis
  • Growth Hormone/genetics
  • Mutation
  • Homeodomain Proteins/biosynthesis
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/physiology
  • Protein Tyrosine Phosphatases/genetics
  • Protein Tyrosine Phosphatases/physiology*
  • Amino Acid Sequence
  • Nuclear Proteins/genetics
  • Nuclear Proteins/physiology*
  • Pituitary Gland, Anterior/cytology*
  • Pituitary Gland, Anterior/embryology*
  • Zebrafish Proteins/biosynthesis
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • Animals
  • Cell Lineage/genetics
  • Cell Lineage/physiology*
  • LIM-Homeodomain Proteins
  • Molecular Sequence Data
  • Apoptosis/genetics
  • Apoptosis/physiology
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Gene Expression Regulation, Developmental/physiology
  • Cell Differentiation/genetics
  • Cell Differentiation/physiology*
  • Thyrotropin/biosynthesis
  • Thyrotropin/genetics
  • Intracellular Signaling Peptides and Proteins/genetics
  • Intracellular Signaling Peptides and Proteins/physiology*
  • Transcription Factor Pit-1/biosynthesis
  • Transcription Factor Pit-1/genetics
(all 42)
PubMed
16458879 Full text @ Dev. Biol.
Abstract
The homeodomain transcription factor Six1 and its modulator, the protein phosphatase Eya1, cooperate to promote cell differentiation and survival during mouse organ development. Here, we studied the effects caused by loss of eya1 and six1 function on pituitary development in zebrafish. eya1 and six1 are co-expressed in all adenohypophyseal cells. Nevertheless, eya1 (aal, dog) mutants show lineage-specific defects, defining corticotropes, melanotropes, and gonadotropes as an Eya1-dependent lineage, which is complementary to the Pit1 lineage. Furthermore, eya1 is required for maintenance of pit1 expression, leading to subsequent loss of cognate hormone gene expression in thyrotropes and somatotropes of mutant embryos, whereas prolactin expression in lactotropes persists. In contrast to other organs, adenohypophyseal cells of eya1 mutants do not become apoptotic, and the adenohypophysis remains at rather normal size. Also, cells do not trans-differentiate, as in the case of pit1 mutants, but display morphological features characteristic for nonsecretory cells. Some of the adenohypophyseal defects of eya1 mutants are moderately enhanced in combination with antisense-mediated loss of Six1 function, which per se does not affect pituitary cell differentiation. In conclusion, this is the first report of an essential role of Eya1 during pituitary development in vertebrates. Eya1 is required for lineage-specific differentiation of adenohypophyseal cells, but not for their survival, thereby uncoupling the differentiation-promoting and anti-apoptotic effects of Eya proteins seen in other tissues.
Genes / Markers
Figures
Figure Gallery (10 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
eya1t22744/t22744standard conditionsUnknown
eya1t22744/t22744standard conditionsPrim-5
eya1t22744/t22744standard conditionsPrim-5
eya1t22744/t22744standard conditionsPrim-15
eya1t22744/t22744standard conditionsProtruding-mouth
eya1t22744/t22744standard conditionsDay 5
eya1t22744/t22744standard conditionsDay 5
eya1t22744/t22744standard conditionsDay 6
eya1t22744/t22744standard conditionsDays 7-13
eya1t22744/t22744standard conditionsDays 7-13
1 - 10 of 20
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
t4
    		Deficiency
    t21142
      		Point Mutation
      t22744
        		Point Mutation
        tm90b
          		Point Mutation
          1 - 4 of 4
          Show
          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          pou1f1MO1-pou1f1MRPHLNO
          six1bMO1-six1bMRPHLNO
          six1bMO2-six1bMRPHLNO
          1 - 3 of 3
          Show
          Fish
          Fish
          Df(Chr07)t4/t4
          eya1t22744/t22744
          eya1tm90b/tm90b
          eya1tm90b/tm90b + MO1-pou1f1
          eya1tm90b/tm90b + MO1-six1b
          fgf3t21142/t21142 (TU)
          WT
          WT + MO1-pou1f1
          WT + MO1-six1b
          1 - 9 of 9
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          Antibodies
          No data available
          Orthology
          No data available
          Engineered Foreign Genes
          No data available
          Mapping
          Entity Type Entity Symbol Location
          Featuret22744Chr: 24 Details
          GENEeya1Chr: 24 Details
          SSLPz7823Chr: 24 Details
          SSLPz24856Chr: 24 Details
          1 - 4 of 4
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          Citation
          Nica, G., Herzog, W., Sonntag, C., Nowak, M., Schwarz, H., Zapata, A.G., Hammerschmidt, M. (2006) Eya1 is required for lineage-specific differentiation, but not for cell survival in the zebrafish adenohypophysis. Developmental Biology. 292(1):189-204.