|ZFIN ID: ZDB-PUB-060210-1|
Molecular cloning, expression and characterization of the zebrafish bram1 gene, a BMP receptor-associated molecule
Wu, K.M., Huang, C.J., Hwang, S.P., and Chang, Y.S.
|Source:||Journal of Biomedical Science 13(3): 345-355 (Journal)|
|Registered Authors:||Huang, Chang-Jen, Hwang, Sheng-Ping L.|
|Keywords:||BRAM1 - BMPRIA - whole-mount in situ hybridization - real time RT-PCR - zebrafish - BMP signaling|
|PubMed:||16456708 Full text @ J. Biomed. Sci.|
Wu, K.M., Huang, C.J., Hwang, S.P., and Chang, Y.S. (2006) Molecular cloning, expression and characterization of the zebrafish bram1 gene, a BMP receptor-associated molecule. Journal of Biomedical Science. 13(3):345-355.
ABSTRACTWe have identified a cDNA clone encoding BMP receptor-associated molecule 1 (BRAM1) from the zebrafish expressed sequence tag (EST) database. The 2606 bp full-length bram1 cDNA was cloned, and further confirmed by nucleotide sequencing. The zebrafish sequence encodes a protein of 195 amino acids with an evolutionarily conserved MYND domain, which displays approximately approximately 98% homology with human and mouse BRAM1, and approximately approximately 64% homology with C. elegans BRA-1 and BRA-2. The bram1 gene, composed of five exons and four introns, spans approximately approximately 14 kb on linkage group 14 of the zebrafish genome. RT-PCR and whole mount in situ hybridization analyses disclosed that zebrafish BRAM1 is a maternal factor. The protein interacts directly with zebrafish BMP Receptor type IA, as observed from GST-pull down and co-immunoprecipitation assays. Furthermore, cotransfection of zebrafish BRAM1 with the corresponding BMP receptor resulted in down-regulation of BMP-mediated signaling. Our results collectively indicate that BRAM1 plays a biological role during zebrafish development.