PUBLICATION
Molecular cloning, expression and characterization of the zebrafish bram1 gene, a BMP receptor-associated molecule
- Authors
- Wu, K.M., Huang, C.J., Hwang, S.P., and Chang, Y.S.
- ID
- ZDB-PUB-060210-1
- Date
- 2006
- Source
- Journal of Biomedical Science 13(3): 345-355 (Journal)
- Registered Authors
- Huang, Chang-Jen, Hwang, Sheng-Ping L.
- Keywords
- BRAM1 - BMPRIA - whole-mount in situ hybridization - real time RT-PCR - zebrafish - BMP signaling
- MeSH Terms
-
- 3T3 Cells
- Amino Acid Sequence
- Animals
- Base Sequence
- Bone Morphogenetic Proteins/metabolism*
- COS Cells
- Caenorhabditis elegans
- Carrier Proteins/chemistry*
- Carrier Proteins/genetics*
- Chlorocebus aethiops
- Cloning, Molecular
- Expressed Sequence Tags
- Humans
- Mice
- Molecular Sequence Data
- Sequence Homology, Amino Acid
- Zebrafish
- Zebrafish Proteins/chemistry*
- Zebrafish Proteins/genetics*
- PubMed
- 16456708 Full text @ J. Biomed. Sci.
Citation
Wu, K.M., Huang, C.J., Hwang, S.P., and Chang, Y.S. (2006) Molecular cloning, expression and characterization of the zebrafish bram1 gene, a BMP receptor-associated molecule. Journal of Biomedical Science. 13(3):345-355.
Abstract
We have identified a cDNA clone encoding BMP receptor-associated molecule 1 (BRAM1) from the zebrafish expressed sequence tag (EST) database. The 2606 bp full-length bram1 cDNA was cloned, and further confirmed by nucleotide sequencing. The zebrafish sequence encodes a protein of 195 amino acids with an evolutionarily conserved MYND domain, which displays approximately approximately 98% homology with human and mouse BRAM1, and approximately approximately 64% homology with C. elegans BRA-1 and BRA-2. The bram1 gene, composed of five exons and four introns, spans approximately approximately 14 kb on linkage group 14 of the zebrafish genome. RT-PCR and whole mount in situ hybridization analyses disclosed that zebrafish BRAM1 is a maternal factor. The protein interacts directly with zebrafish BMP Receptor type IA, as observed from GST-pull down and co-immunoprecipitation assays. Furthermore, cotransfection of zebrafish BRAM1 with the corresponding BMP receptor resulted in down-regulation of BMP-mediated signaling. Our results collectively indicate that BRAM1 plays a biological role during zebrafish development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping