PUBLICATION

Proteomics of early zebrafish embryos

Authors
Link, V., Shevchenko, A., and Heisenberg, C.P.
ID
ZDB-PUB-060124-16
Date
2006
Source
BMC Developmental Biology   6: 1 (Journal)
Registered Authors
Heisenberg, Carl-Philipp, Link, Vinzenz
Keywords
none
MeSH Terms
  • Animals
  • Blotting, Western
  • Databases, Protein
  • Egg Yolk/chemistry
  • Electrophoresis, Gel, Two-Dimensional
  • Embryo, Nonmammalian/chemistry
  • Embryo, Nonmammalian/metabolism
  • Mass Spectrometry
  • Proteomics/methods*
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Zebrafish Proteins/analysis*
  • Zebrafish Proteins/metabolism
PubMed
16412219 Full text @ BMC Dev. Biol.
Abstract
BACKGROUND: Zebrafish (D. rerio) has become a powerful and widely used model system for the analysis of vertebrate embryogenesis and organ development. While genetic methods are readily available in zebrafish, protocols for two dimensional (2D) gel electrophoresis and proteomics have yet to be developed.
RESULTS: As a prerequisite to carry out proteomic experiments with early zebrafish embryos, we developed a method to efficiently remove the yolk from large batches of embryos. This method enabled high resolution 2D gel electrophoresis and improved Western blotting considerably. Here, we provide detailed protocols for proteomics in zebrafish from sample preparation to mass spectrometry (MS), including a comparison of databases for MS identification of zebrafish proteins.
CONCLUSIONS: The provided protocols for proteomic analysis of early embryos enable research to be taken in novel directions in embryogenesis.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes