ZFIN ID: ZDB-PUB-060124-16
Proteomics of early zebrafish embryos
Link, V., Shevchenko, A., and Heisenberg, C.P.
Date: 2006
Source: BMC Developmental Biology   6: 1 (Journal)
Registered Authors: Heisenberg, Carl-Philipp, Link, Vinzenz
Keywords: none
MeSH Terms:
  • Animals
  • Blotting, Western
  • Databases, Protein
  • Egg Yolk/chemistry
  • Electrophoresis, Gel, Two-Dimensional
  • Embryo, Nonmammalian/chemistry
  • Embryo, Nonmammalian/metabolism
  • Mass Spectrometry
  • Proteomics/methods*
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Zebrafish Proteins/analysis*
  • Zebrafish Proteins/metabolism
PubMed: 16412219 Full text @ BMC Dev. Biol.
BACKGROUND: Zebrafish (D. rerio) has become a powerful and widely used model system for the analysis of vertebrate embryogenesis and organ development. While genetic methods are readily available in zebrafish, protocols for two dimensional (2D) gel electrophoresis and proteomics have yet to be developed.
RESULTS: As a prerequisite to carry out proteomic experiments with early zebrafish embryos, we developed a method to efficiently remove the yolk from large batches of embryos. This method enabled high resolution 2D gel electrophoresis and improved Western blotting considerably. Here, we provide detailed protocols for proteomics in zebrafish from sample preparation to mass spectrometry (MS), including a comparison of databases for MS identification of zebrafish proteins.
CONCLUSIONS: The provided protocols for proteomic analysis of early embryos enable research to be taken in novel directions in embryogenesis.