PUBLICATION
Identification of two proopiomelanocortin genes in zebrafish (Danio rerio)
- Authors
- Gonzalez-Nuñez, V., Gonzalez-Sarmiento, R., and Rodriguez, R.E.
- ID
- ZDB-PUB-051214-6
- Date
- 2003
- Source
- Brain research. Molecular brain research 120(1): 1-8 (Journal)
- Registered Authors
- González Nuñez, Veronica, Rodriguez, Raquel E.
- Keywords
- Zebrafish; Opioids; Proopiomelanocortin; beta-Endorphin
- MeSH Terms
-
- Zebrafish Proteins/biosynthesis
- Zebrafish Proteins/genetics*
- Brain/metabolism
- Zebrafish
- Animals
- alpha-MSH/genetics
- Base Sequence
- Conserved Sequence
- Phylogeny
- Adrenocorticotropic Hormone/genetics
- Reverse Transcriptase Polymerase Chain Reaction/methods
- Cloning, Molecular
- Pituitary Gland/metabolism
- Pro-Opiomelanocortin/biosynthesis
- Pro-Opiomelanocortin/classification
- Pro-Opiomelanocortin/genetics*
- beta-Endorphin/genetics
- beta-Lipotropin/genetics
- RNA, Messenger/biosynthesis
- Exons
- Introns
- Sequence Alignment
- Amino Acid Sequence
- PubMed
- 14667571 Full text @ Brain Res. Mol. Brain Res.
Citation
Gonzalez-Nuñez, V., Gonzalez-Sarmiento, R., and Rodriguez, R.E. (2003) Identification of two proopiomelanocortin genes in zebrafish (Danio rerio). Brain research. Molecular brain research. 120(1):1-8.
Abstract
Characterization of a newly cloned proopiomelanocortin (POMC) gene in the teleost zebrafish, Danio rerio, is reported. This gene is formed by three exons and two introns, and its complete cDNA codes for a polypeptide of 222 amino acids. Zebrafish proopiomelanocortin (zfPOMC) contains the consensus sequences for ACTH, gamma-LPH, beta-MSH and beta-endorphin (beta-END). RT-PCR expression studies indicate that zfPOMC is selectively expressed in nervous tissue and in the pituitary gland. An homologous sequence to zfPOMC in the zebrafish genome is also presented. It is possible that this sequence represents part of a duplicate POMC, which might have appeared as a result of an extra genome duplication that have taken place in the cyprinidae family or even in all teleosts. Comparisons between the two zebrafish beta-endorphins and among these peptides and its homologues in other species are also presented.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping