PUBLICATION
Bone morphogenetic protein 15 in the zebrafish ovary: cDNA cloning, genomic organization, tissue distribution and role in oocyte maturation
- Authors
- Clelland, E., Kohli, G., Campbell, R.K., Sharma, S., Shimasaki, S., and Peng, C.
- ID
- ZDB-PUB-051012-15
- Date
- 2006
- Source
- Endocrinology 147(1): 201-209 (Journal)
- Registered Authors
- Campbell, Robert K., Peng, Chun
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Bone Morphogenetic Protein 15
- Chickens
- Cloning, Molecular
- DNA Primers
- DNA, Complementary/genetics*
- Female
- Growth Differentiation Factor 9
- Humans
- Intercellular Signaling Peptides and Proteins/genetics*
- Mammals
- Molecular Sequence Data
- Oocytes/physiology*
- Organ Specificity
- Phylogeny
- Polymerase Chain Reaction
- Zebrafish/classification
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- PubMed
- 16210364 Full text @ Endocrinology
Citation
Clelland, E., Kohli, G., Campbell, R.K., Sharma, S., Shimasaki, S., and Peng, C. (2006) Bone morphogenetic protein 15 in the zebrafish ovary: cDNA cloning, genomic organization, tissue distribution and role in oocyte maturation. Endocrinology. 147(1):201-209.
Abstract
Bone morphogenetic protein-15 (BMP-15) is a member of the transforming growth factor-beta family known to regulate ovarian functions in mammals. The structure and function of BMP-15 in lower vertebrates are less known. In this study, we cloned the zebrafish BMP-15 (zfBMP-15) cDNA and depicted its genomic organization. The zfBMP-15 cDNA encodes a protein of 384 amino acids. The mature protein has 46-51% sequence identities to fugu, chicken, and mammalian BMP-15. It also shares 38-46% homology with growth and differentiation factor (GDF)-9 in fishes, chicken and mammals. Phylogenetic analysis further confirms that the zfBMP-15 is most closely related to BMP-15 from other species while the GDF-9 peptides from fish to mammals form a distinct branch. Comparison of zfBMP-15 cDNA with zebrafish genome database revealed that zfBMP-15 is encoded by a gene with two exons and one intron, located on chromosome 6. BMP-15 mRNA is expressed in the ovary and testis, and to a lesser extent, in brain, liver, gut, heart and muscle. Real-time PCR revealed that BMP-15 is expressed in follicles at all stages of development with no significant changes over the course of folliculogenesis. Using in situ hybridization and immunocytochemistry, we detected BMP-15 in both oocytes and follicular cells. Incubation of follicles with antiserum against zfBMP15 increased oocyte maturation, while incubation with recombinant human BMP-15 suppressed human chorionic gonadotropin-induced oocyte maturation. These findings suggest that BMP-15 plays a role in regulating gonadal functions in fish, in particular oocyte maturation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping