PUBLICATION
Glucagon-like peptide-1 activates the adenylyl cyclase system in rockfish enterocytes and brain membranes
- Authors
- Mommsen, T.P., and Mojsov, S.
- ID
- ZDB-PUB-051007-1
- Date
- 1998
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 121(1): 49-56 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Enzyme Activation/drug effects
- Liver/drug effects
- Liver/metabolism
- Brain/drug effects
- Brain/metabolism
- Mammals/metabolism
- Peptide Fragments/metabolism
- Peptide Fragments/pharmacology*
- Fishes/metabolism*
- Glucagon/metabolism
- Glucagon/pharmacology*
- Membranes/drug effects
- Membranes/metabolism
- Signal Transduction
- Glucagon-Like Peptide 1
- Cyclic AMP/metabolism
- Protein Precursors/metabolism
- Protein Precursors/pharmacology*
- In Vitro Techniques
- Adenylyl Cyclases/metabolism*
- Intestines/drug effects
- Intestines/metabolism
- PubMed
- 9972283 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Mommsen, T.P., and Mojsov, S. (1998) Glucagon-like peptide-1 activates the adenylyl cyclase system in rockfish enterocytes and brain membranes. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 121(1):49-56.
Abstract
Glucagon-like peptide (GLP) exerts important physiological functions in fish liver, but extrahepatic sites of action and physiological roles have been largely ignored. We show here that GLP activates adenylyl cyclase in isolated brain and enterocyte membranes and increases cellular cyclic adenosine monophosphate (cAMP) levels in isolated enterocytes of rockfish (Sebastes caurinus). Following exposure to synthetic zebrafish GLP (zf-GLP) (1 nM-1 microM), a concentration-dependent increase in enterocyte cAMP is noted. The maximum increase in cAMP levels is observed at 1 microM zf-GLP, and represents a 30% increase above control values. Exendin-4, a GLP receptor agonist in mammals, elicits a similar concentration-dependent increase in enterocyte cAMP. In contrast, norepinephrine or prostaglandin E2 (at 1 microM) increased cAMP levels by 2 and 4-fold, respectively. Brain membrane adenylyl cyclase is activated 20-40% by zf-GLP, and to a smaller extent by zf-glucagon, while exendin-4 is as effective as zf-GLP at a dose of 100 nM. These results suggest potential physiological roles of GLP in brain and intestine in piscine systems analogous to GLP-1 functions in these tissues described for mammals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping