PUBLICATION
Wnt11 Functions in Gastrulation by Controlling Cell Cohesion through Rab5c and E-Cadherin
- Authors
- Ulrich, F., Krieg, M., Schotz, E.M., Link, V., Castanon, I., Schnabel, V., Taubenberger, A., Mueller, D., Puech, PH., and Heisenberg, C.P.
- ID
- ZDB-PUB-051005-6
- Date
- 2005
- Source
- Developmental Cell 9(4): 555-564 (Journal)
- Registered Authors
- Heisenberg, Carl-Philipp, Link, Vinzenz, Ulrich, Florian
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cadherins/genetics
- Cadherins/metabolism*
- Cell Adhesion
- Cell Movement/physiology
- Endocytosis/physiology
- Gastrula/cytology
- Gastrula/physiology*
- Morphogenesis
- Protein Isoforms/genetics
- Protein Isoforms/metabolism
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/metabolism
- Signal Transduction/physiology
- Wnt Proteins/genetics
- Wnt Proteins/metabolism*
- Zebrafish/anatomy & histology
- Zebrafish/embryology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- rab5 GTP-Binding Proteins/genetics
- rab5 GTP-Binding Proteins/metabolism*
- PubMed
- 16198297 Full text @ Dev. Cell
Citation
Ulrich, F., Krieg, M., Schotz, E.M., Link, V., Castanon, I., Schnabel, V., Taubenberger, A., Mueller, D., Puech, PH., and Heisenberg, C.P. (2005) Wnt11 Functions in Gastrulation by Controlling Cell Cohesion through Rab5c and E-Cadherin. Developmental Cell. 9(4):555-564.
Abstract
Wnt11 plays a central role in tissue morphogenesis during vertebrate gastrulation, but the molecular and cellular mechanisms by which Wnt11 exerts its effects remain poorly understood. Here, we show that Wnt11 functions during zebrafish gastrulation by regulating the cohesion of mesodermal and endodermal (mesendodermal) progenitor cells. Importantly, we demonstrate that Wnt11 activity in this process is mediated by the GTPase Rab5, a key regulator of early endocytosis, as blocking Rab5c activity in wild-type embryos phenocopies slb/wnt11 mutants, and enhancing Rab5c activity in slb/wnt11 mutant embryos rescues the mutant phenotype. In addition, we find that Wnt11 and Rab5c control the endocytosis of E-cadherin and are required in mesendodermal cells for E-cadherin-mediated cell cohesion. Together, our results suggest that Wnt11 controls tissue morphogenesis by modulating E-cadherin-mediated cell cohesion through Rab5c, a novel mechanism of Wnt signaling in gastrulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping