PUBLICATION
Transcriptional regulation and O-GlcNAcylation activity of zebrafish OGT during embryogenesis
- Authors
- Sohn, K.C., and Do, S.I.
- ID
- ZDB-PUB-050930-10
- Date
- 2005
- Source
- Biochemical and Biophysical Research Communications 337(1): 256-263 (Journal)
- Registered Authors
- Keywords
- zO-GlcNAc transferase, Transcriptional variants, O-GlcNAc, Developmental regulation
- MeSH Terms
-
- Alternative Splicing
- Amino Acid Sequence
- Animals
- Base Sequence
- Embryonic Development
- Escherichia coli/genetics
- Gene Expression Regulation, Developmental
- Genetic Variation
- Molecular Sequence Data
- N-Acetylglucosaminyltransferases/classification
- N-Acetylglucosaminyltransferases/genetics*
- N-Acetylglucosaminyltransferases/metabolism*
- Phylogeny
- Sequence Alignment
- Transcription, Genetic
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/classification
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism*
- PubMed
- 16188232 Full text @ Biochem. Biophys. Res. Commun.
Citation
Sohn, K.C., and Do, S.I. (2005) Transcriptional regulation and O-GlcNAcylation activity of zebrafish OGT during embryogenesis. Biochemical and Biophysical Research Communications. 337(1):256-263.
Abstract
Zebrafish OGT (zOGT) sequence was identified in zebrafish (Danio rerio) genome and six different transcriptional variants of zOGT, designated var1 to var6, were isolated. Here we describe the developmental regulation of zOGT variants at transcriptional level and characterization of their OGT activities of protein O-GlcNAcylation. OGT transcriptional variants in zebrafish were differentially generated by alternative splicing and in particular, var1 and var2 were contained by 48 bp intron as a novel exon sequence, demonstrating that this form of OGT was not found in mammals. Transcript analysis revealed that var1 and var2 were highly expressed at early phase of development including unfertilized egg until dome stage whereas var3 and var4 were begins to be expressed at sphere stage until late phase of development. Our data indicate that var1 and var2 are likely to be maternal transcripts. The protein expression assay in Escherichia coli-p62 system showed that OGT activities of var3 and var4 were found to be only active whereas those of other variants were inactive.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping