PUBLICATION
Initiation of convergence and extension movements of lateral mesoderm during zebrafish gastrulation
- Authors
- Sepich, D.S., Calmelet, C., Kiskowski, M., and Solnica-Krezel, L.
- ID
- ZDB-PUB-050831-11
- Date
- 2005
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 234(2): 279-292 (Journal)
- Registered Authors
- Sepich, Diane, Solnica-Krezel, Lilianna
- Keywords
- convection, migration, directional cues, turning, noncanonical Wnt, Stat3
- MeSH Terms
-
- Movement
- Wnt Proteins/metabolism
- Gene Expression Regulation*
- Wnt3 Protein
- STAT3 Transcription Factor/metabolism
- Chemotaxis
- Ectoderm/metabolism
- Chemotactic Factors/chemistry
- Time Factors
- Endoderm/metabolism
- Models, Biological
- Mesoderm/metabolism
- Mesoderm/pathology*
- Animals
- Cell Movement
- Models, Genetic
- Gastrula/metabolism
- Gastrula/pathology*
- Zebrafish
- Signal Transduction
- PubMed
- 16127722 Full text @ Dev. Dyn.
Citation
Sepich, D.S., Calmelet, C., Kiskowski, M., and Solnica-Krezel, L. (2005) Initiation of convergence and extension movements of lateral mesoderm during zebrafish gastrulation. Developmental Dynamics : an official publication of the American Association of Anatomists. 234(2):279-292.
Abstract
Embryonic morphogenesis is accomplished by cellular movements, rearrangements, and cell fate inductions. Vertebrate gastrulation entails morphogenetic processes that generate three germ layers, endoderm, mesoderm, and ectoderm, shaped into head, trunk, and tail. To understand how cell migration mechanistically contributes to tissue shaping during gastrulation, we examined migration of lateral mesoderm in the zebrafish. Our results illustrate that cell behaviors, different from mediolaterally oriented cell intercalation, also promote convergence and extension (C&E). During early gastrulation, upon internalization, individually migrating mesendodermal cells contribute to the elongation of the mesoderm by moving animally, without dorsal movement. Convergence toward dorsal starts later, by 70% epiboly (7.7 hpf). Depending on location along the Animal-Vegetal axis, an animal or vegetal bias is added to the dorsalward movement, so that paths fan out and the lateral mesoderm both converges and extends. Onset of convergence is independent of noncanonical Wnt signaling but is delayed when Stat3 signaling is compromised. To understand which aspects of motility are controlled by guidance cues, we measured turning behavior of lateral mesodermal cells. We show that cells exhibit directional preference, directionally-regulated speed, and turn toward dorsal when off-course. We estimate that ectoderm could supply from a fraction to all the dorsalward displacement seen in mesoderm cells. Using mathematical modeling, we demonstrate that directional preference is sufficient to account for mesoderm convergence and extension, and that, at minimum, two sources of guidance cues could orient cell paths realistically if located in the dorsal midline.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping