PUBLICATION
Fluorosis: a new model and new insights
- Authors
- Bartlett, J.D., Dwyer, S.E., Beniash, E., Skobe, Z., and Payne-Ferreira, T.L.
- ID
- ZDB-PUB-050823-7
- Date
- 2005
- Source
- Journal of dental research 84(9): 832-836 (Journal)
- Registered Authors
- Payne-Ferreira, Tracie
- Keywords
- apoptosis, cell signaling, fluorosis, tooth development, zebrafish
- MeSH Terms
-
- Activin Receptors, Type I/biosynthesis
- Ameloblasts/pathology
- Animals
- Cariostatic Agents/analysis
- Cariostatic Agents/toxicity*
- Dental Enamel/chemistry*
- Dental Enamel/pathology
- Down-Regulation
- Fluorides/analysis
- Fluorides/toxicity*
- Fluorosis, Dental/metabolism
- Fluorosis, Dental/pathology*
- Immunohistochemistry
- Microscopy, Electron, Scanning
- Models, Animal*
- Spectroscopy, Fourier Transform Infrared
- Zebrafish*
- Zebrafish Proteins/biosynthesis
- PubMed
- 16109993 Full text @ J. Dent. Res.
Citation
Bartlett, J.D., Dwyer, S.E., Beniash, E., Skobe, Z., and Payne-Ferreira, T.L. (2005) Fluorosis: a new model and new insights. Journal of dental research. 84(9):832-836.
Abstract
Fluoride is an effective agent for the prevention of dental caries. However, the mechanism of how excessive fluoride exposure causes fluorosis remains uncertain. Zebrafish (Danio rerio) exhibit periodic tooth replacement throughout their lives, thereby providing continuous access to teeth at developmental stages susceptible to fluoride exposure. Zebrafish teeth do not contain true enamel, but consist of a hard enameloid surface. Therefore, we asked whether zebrafish could be used as a model organism for the study of dental fluorosis. Scanning electron microscopy of fluoride-treated teeth demonstrated that the enameloid was pitted and rough, and FTIR analysis demonstrated that the teeth also contained a significantly higher organic content when compared with untreated controls. Furthermore, we demonstrate for the first time that decreased expression of an important signaling molecule (Alk8) in tooth development may contribute to the observed fluorotic phenotype, and that increased cell apoptosis may also play a role in the mechanism of fluorosis
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping