PUBLICATION
Molecular cloning of the BCL-6 gene, a transcriptional repressor for B-cell differentiation, in torafugu (Takifugu rubripes)
- Authors
- Ohtani, M., Miyadai, T., and Hiroishi, S.
- ID
- ZDB-PUB-050818-18
- Date
- 2006
- Source
- Molecular immunology 43(7): 1047-1053 (Journal)
- Registered Authors
- Keywords
- BCL-6, Transcription factor, Torafugu, B-cell
- MeSH Terms
-
- Cloning, Molecular
- Amino Acid Sequence
- RNA, Messenger/analysis
- Introns/genetics
- Proto-Oncogene Proteins c-bcl-6/chemistry
- Proto-Oncogene Proteins c-bcl-6/genetics*
- Cell Differentiation/genetics
- B-Lymphocytes/cytology
- B-Lymphocytes/physiology
- Base Sequence
- Amino Acid Motifs/genetics
- Takifugu/genetics*
- Takifugu/metabolism
- Exons/genetics
- Transcription, Genetic*
- Zinc Fingers/genetics
- Molecular Sequence Data
- Animals
- Repressor Proteins/chemistry
- Repressor Proteins/genetics*
- PubMed
- 16076494 Full text @ Mol. Immunol.
Citation
Ohtani, M., Miyadai, T., and Hiroishi, S. (2006) Molecular cloning of the BCL-6 gene, a transcriptional repressor for B-cell differentiation, in torafugu (Takifugu rubripes). Molecular immunology. 43(7):1047-1053.
Abstract
B-cell lymphoma-6 (BCL-6) is a transcriptional repressor that prevents the terminal differentiation of mature B-cells to plasma cells, and is essential for germinal center formation in the primary lymphoid organs of mammals. In this study, we identified the BCL-6 gene in torafugu (Takifugu rubripes) using the torafugu genome database, and analyzed the expression of BCL-6 mRNA in various tissues of torafugu, using RT-PCR. The BCL-6 gene consisted of eight exons and seven introns spanning a genome of ca. 3.3kb. BCL-6 mRNA contained a 2112bp open reading frame encoding 703 amino acids, with a predicted protein size of 78.8kDa. The predicted torafugu BCL-6 primary structure contains two conserved specific motifs, the BTB/POZ domain at the N-terminus and the sixC(2)H(2)-type zinc finger motifs at the C-terminal region. The homology of torafugu BCL-6 to those of zebrafish (Danio rerio), Xenopus laevis, mouse (Mus musculus) and human (Homo sapiens) is 76, 59, 60 and 60%, respectively. RT-PCR analysis revealed that BCL-6 mRNA is highly expressed in pronephros, thymus, intestine, ovary, brain, nasal cavity and muscle. These results imply that torafugu BCL-6 is involved in regulation of B-cell differentiation in torafugu.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping