PUBLICATION
Gata4 regulates the formation of multiple organs
- Authors
- Holtzinger, A., and Evans, T.
- ID
- ZDB-PUB-050810-6
- Date
- 2005
- Source
- Development (Cambridge, England) 132(17): 4005-4014 (Journal)
- Registered Authors
- Evans, Todd
- Keywords
- Organogenesis, Heart, Liver, Pancreas, Zebrafish
- MeSH Terms
-
- Animals
- DNA-Binding Proteins/deficiency
- DNA-Binding Proteins/genetics*
- DNA-Binding Proteins/metabolism*
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- GATA4 Transcription Factor
- GATA6 Transcription Factor
- Gastrointestinal Tract/embryology
- Gastrointestinal Tract/metabolism
- Gene Expression
- Heart/embryology
- Models, Biological
- Myocardium/metabolism
- Organogenesis*/genetics
- Transcription Factors/deficiency
- Transcription Factors/genetics*
- Transcription Factors/metabolism*
- Zebrafish/anatomy & histology
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 16079152 Full text @ Development
Citation
Holtzinger, A., and Evans, T. (2005) Gata4 regulates the formation of multiple organs. Development (Cambridge, England). 132(17):4005-4014.
Abstract
We have developed a loss-of-function model for Gata4 in zebrafish, in order to examine broadly its requirement for organogenesis. We show that the function of Gata4 in zebrafish heart development is well conserved with that in mouse, and that, in addition, Gata4 is required for development of the intestine, liver, pancreas and swim bladder. Therefore, a single transcription factor regulates the formation of many organs. Gata6 is a closely related transcription factor with an overlapping expression pattern. We show that zebrafish depleted of Gata6 show defects in liver bud growth similar to mouse Gata6 mutants and zebrafish Gata4 morphants, and that zebrafish embryos depleted of both Gata4 and Gata6 display an earlier block in liver development, and thus completely lack liver buds. Therefore, Gata4 and Gata6 have distinct non-redundant functions in cardiac morphogenesis, but are redundant for an early step of liver development. In addition, both Gata4 and Gata6 are essential and non-redundant for liver growth following initial budding.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping