PUBLICATION
An Oct-1 binding site mediates activation of the gata2 promoter by BMP signaling
- Authors
- Oren, T., Torregroza, I., and Evans, T.
- ID
- ZDB-PUB-050803-16
- Date
- 2005
- Source
- Nucleic acids research 33(13): 4357-4367 (Journal)
- Registered Authors
- Evans, Todd
- Keywords
- none
- MeSH Terms
-
- Animals
- Binding Sites
- Bone Morphogenetic Protein 4
- Bone Morphogenetic Proteins/physiology*
- DNA-Binding Proteins/genetics*
- DNA-Binding Proteins/metabolism*
- GATA2 Transcription Factor
- Gene Expression Regulation, Developmental*
- Octamer Transcription Factor-1
- Promoter Regions, Genetic*
- Response Elements*
- Sequence Deletion
- Signal Transduction
- Smad Proteins
- Trans-Activators/metabolism
- Transcription Factors/genetics*
- Transcription Factors/metabolism*
- Transcriptional Activation*
- Xenopus Proteins
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- PubMed
- 16061939 Full text @ Nucleic Acids Res.
Citation
Oren, T., Torregroza, I., and Evans, T. (2005) An Oct-1 binding site mediates activation of the gata2 promoter by BMP signaling. Nucleic acids research. 33(13):4357-4367.
Abstract
The gata2 gene encodes a transcription factor implicated in regulating early patterning of ectoderm and mesoderm, and later in numerous cell-specific gene expression programs. Activation of the gata2 gene during embryogenesis is dependent on the bone morphogenetic protein (BMP) signaling pathway, but the mechanism for how signaling controls gene activity has not been defined. We developed an assay in Xenopus embryos to analyze regulatory sequences of the zebrafish gata2 promoter that are necessary to mediate the response to BMP signaling during embryogenesis. We show that activation is Smad dependent, since it is blocked by expression of the inhibitory Smad6. Deletion analysis identified an octamer binding site that is necessary for BMP-mediated induction, and that interacts with the POU homeodomain protein Oct-1. However, this element is not sufficient to transfer a BMP response to a heterologous promoter, requiring an additional more proximal cooperating element. Based on recent studies with other BMP-dependent promoters (Drosophila vestigial and Xenopus Xvent-2), our studies of the gata2 gene suggest that POU-domain proteins comprise a common component of the BMP signaling pathway, cooperating with Smad proteins and other transcriptional activators.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping