PUBLICATION
Inositol polyphosphates regulate zebrafish left-right asymmetry
- Authors
- Sarmah, B., Latimer, A.J., Appel, B., and Wente, S.R.
- ID
- ZDB-PUB-050711-3
- Date
- 2005
- Source
- Developmental Cell 9(1): 133-145 (Journal)
- Registered Authors
- Appel, Bruce, Latimer, Andrew
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Body Patterning*
- Calcium Signaling
- Embryo, Nonmammalian/metabolism
- Molecular Sequence Data
- Phosphotransferases (Alcohol Group Acceptor)/genetics
- Phosphotransferases (Alcohol Group Acceptor)/metabolism*
- Phytic Acid/biosynthesis
- Sequence Homology, Amino Acid
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 15992547 Full text @ Dev. Cell
Citation
Sarmah, B., Latimer, A.J., Appel, B., and Wente, S.R. (2005) Inositol polyphosphates regulate zebrafish left-right asymmetry. Developmental Cell. 9(1):133-145.
Abstract
Vertebrate body plans have a conserved left-right (LR) asymmetry manifested in the position and anatomy of the heart, visceral organs, and brain. Recent studies have suggested that LR asymmetry is established by asymmetric Ca(2+) signaling resulting from cilia-driven flow of extracellular fluid across the node. We report here that inositol 1,3,4,5,6-pentakisphosphate 2-kinase (Ipk1), which generates inositol hexakisphosphate, is critical for normal LR axis determination in zebrafish. Zebrafish embryos express ipk1 symmetrically during gastrulation and early segmentation. ipk1 knockdown by antisense morpholino oligonucleotide injection randomized LR-specific gene expression and organ placement, effects that were associated with reduced intracellular Ca(2+) flux in cells surrounding the ciliated Kupffer's vesicle, a structure analogous to the mouse node. Our data suggest that the pathway for inositol hexakisphosphate production is a key regulator of asymmetric Ca(2+) flux during LR specification.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping