PUBLICATION
Characterization of calpastatin gene in fish: Its potential role in muscle growth and fillet quality
- Authors
- Salem, M., Yao, J., Rexroad, C.E., Kenney, P.B., Semmens, K., Killefer, J., and Nath, J.
- ID
- ZDB-PUB-050711-2
- Date
- 2005
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 141(4): 488-497 (Journal)
- Registered Authors
- Keywords
- Calpastatin; Gene expression; Muscle growth; Fillet quality; Rainbow trout
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Calcium-Binding Proteins/genetics*
- Calcium-Binding Proteins/physiology*
- DNA, Complementary/genetics
- Fishes
- Gene Expression Regulation, Developmental/physiology
- Mice
- Molecular Sequence Data
- Muscle, Skeletal/chemistry
- Muscle, Skeletal/growth & development*
- Muscle, Skeletal/physiology
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Alignment
- Sequence Homology, Amino Acid
- Species Specificity
- PubMed
- 15990347 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Salem, M., Yao, J., Rexroad, C.E., Kenney, P.B., Semmens, K., Killefer, J., and Nath, J. (2005) Characterization of calpastatin gene in fish: Its potential role in muscle growth and fillet quality. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 141(4):488-497.
Abstract
Calpastatin (CAST), the specific inhibitor of the calpain proteases, plays a role in muscle growth and meat quality. In rainbow trout (RBT), we identified cDNAs coding for two CAST isoforms, a long (CAST-L) and a short isoform (CAST-S), apparently derived from two different genes. Zebrafish and pufferfish CAST cDNA and genomic sequences were retrieved from GenBank and their exon/intron structures were characterized. Fish CASTs are novel in that they have fewer repetitive inhibitory domains as compared to their mammalian counterparts (one or two vs. four). The expressions of CAST mRNAs were measured in three RBT strains with different growth rates and fillet firmness that were fed either high energy or control diets. CAST-L and S expressions were significantly lower (p<0.01) in the strain that has the slowest growth rate and yielded the softest fillet. Strain or diet did not affect level of calpain mRNAs. However, the decrease in the CAST/calpain ratio at the mRNA level did not lead to a corresponding change in the calpain catalytic activity. Further investigation should reveal a potential use of the CAST gene as a tool to monitor fish muscle growth and fillet firmness.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping