PUBLICATION
Molecular cloning and expression of a novel CYP26 gene (cyp26d1) during zebrafish early development
- Authors
- Gu, X., Xu, F., Wang, X., Gao, X., and Zhao, Q.
- ID
- ZDB-PUB-050701-8
- Date
- 2005
- Source
- Gene expression patterns : GEP 5(6): 733-739 (Journal)
- Registered Authors
- Wang, Xiaolin, Xu, Fang, Zhao, Qingshun
- Keywords
- Zebrafish; cyp26; cyp26a1; cyp26b1; cyp26d1; Cytochrome P450; Retinoid signaling; Development; Hindbrain; Rhombomere; Eye; Otic vesicle; Pharyngeal arch
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Body Patterning
- Cloning, Molecular
- Conserved Sequence
- Cytochrome P-450 Enzyme System/genetics*
- DNA, Complementary/genetics
- Embryo, Nonmammalian/enzymology
- Gene Expression Regulation, Developmental*
- Gene Expression Regulation, Enzymologic
- Molecular Sequence Data
- Morphogenesis
- Sequence Alignment
- Sequence Homology, Amino Acid
- Zebrafish/embryology*
- Zebrafish Proteins/genetics*
- PubMed
- 15979416 Full text @ Gene Expr. Patterns
Citation
Gu, X., Xu, F., Wang, X., Gao, X., and Zhao, Q. (2005) Molecular cloning and expression of a novel CYP26 gene (cyp26d1) during zebrafish early development. Gene expression patterns : GEP. 5(6):733-739.
Abstract
Proper restriction of retinoid signaling by Cyp26s is essential for development of vertebrate embryos while inappropriate retinoid signaling can cause teratogenesis. Here, we report cloning and expression analysis of a novel cyp26 gene (cyp26d1) isolated from zebrafish. The predicted protein encoded by cyp26d1 consists of 554 amino acids. It exhibits 54% amino acid identity with human Cyp26C1, 50% with zebrafish Cyp26B1 and 38% with zebrafish Cyp26A1. Whole-mount in situ hybridization shows that cyp26d1 is first expressed in sphere stage, then disappears at 50% epiboly and resumes its expression at 75% epiboly. During segmentation period, cyp26d1 message is found at presumptive hindbrain. Double in situ hybridization with krox20 and cyp26d1 reveals that cyp26d1 is expressed in presumptive rhombomere 2-4 (r2-r4) at 2-somite stage. At 3-somite stage, cyp26d1 gene is expressed in r6 and pharyngeal arch (pa) one in addition to its expression at r2 and r4. At 6-somite stage, cyp26d1 message is present in continuous bands at r2-r6 and in pa1. This expression pattern is maintained from 10-somite stage through 21-somite stage except that the expression level is greatly reduced at r2 and r4. At 21-somite stage, cyp26d1 is also found in a group of cells in telencephalon and diencephalons. At 25-31h post-fertilization (hpf), the zebrafish cyp26d1 expression domain is extended to eyes, otic vesicles and midbrain in addition to its expression in hindbrain, telencephalon, diencephalons, and pharyngeal arches. At 35-48hpf, the expression of cyp26d1 is mainly restricted to otic vesicles, pharyngeal arches and pectoral fins and the expression level is greatly reduced.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping