PUBLICATION
Molecular cloning, expression of Sox5 and its down-regulation of Dmrt1 transcription in Zebrafish
- Authors
- Gao, S., Zhang, T., Zhou, X., Zhao, Y., Li, Q., Guo, Y., Cheng, H., and Zhou, R.
- ID
- ZDB-PUB-050701-11
- Date
- 2005
- Source
- Journal of experimental zoology. Part B, Molecular and developmental evolution 304(5): 476-483 (Journal)
- Registered Authors
- Li, Qin
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cloning, Molecular
- Down-Regulation
- Embryo, Nonmammalian
- Female
- Flow Cytometry
- Gene Expression Regulation, Developmental
- Genes, Reporter
- Male
- Molecular Sequence Data
- Promoter Regions, Genetic
- Repressor Proteins/biosynthesis
- Repressor Proteins/genetics*
- Reverse Transcriptase Polymerase Chain Reaction
- SOXD Transcription Factors
- Sequence Homology, Amino Acid
- Sex Differentiation/genetics
- Transcription Factors/biosynthesis*
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish Proteins/biosynthesis
- Zebrafish Proteins/genetics*
- PubMed
- 15981199 Full text @ J. Exp. Zool. B Mol. Dev. Evol.
Citation
Gao, S., Zhang, T., Zhou, X., Zhao, Y., Li, Q., Guo, Y., Cheng, H., and Zhou, R. (2005) Molecular cloning, expression of Sox5 and its down-regulation of Dmrt1 transcription in Zebrafish. Journal of experimental zoology. Part B, Molecular and developmental evolution. 304(5):476-483.
Abstract
The doublesex and mab-3-related transcription factor 1 (Dmrt1) is the founding member of a family of DM domain genes, involved in gonadogenesis. Here we report the cloning of zebrafish Sox5 and use real time PCR to show that its expression increases while expression of Dmrt1 decreases during embryogenesis. Characterization of the proximal promoter of zebrafish Dmrt1 revealed two positive and two negative regulatory regions and a Sox5-binding site. Co-transfection of dmrt1 (with or without the Sox-binding site), driving an EGFP reporter and Sox5 showed further that Sox5 bound the Dmrt1 promoter and inhibited Dmrt1 expression. This antagonistic partnership between Dmrt1 and Sox5 suggests a potential transcriptional regulatory mechanism for Dmrt1 in early embryogenesis. J. Exp. Zool. (Mol. Dev. Evol.) 304B, 2005. (c) 2005 Wiley-Liss, Inc.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping