PUBLICATION

Epithelial Ca2+ Channel Expression and Ca2+ uptake in Developing Zebrafish

Authors
Pan, T.C., Liao, B.K., Huang, C.J., Lin, L.Y., and Hwang, P.P.
ID
ZDB-PUB-050617-1
Date
2005
Source
American journal of physiology. Regulatory, integrative and comparative physiology   289(4): R1202-1211 (Journal)
Registered Authors
Huang, Chang-Jen, Hwang, Pung Pung
Keywords
ECaC, Ca2+ influx, low Ca2+, mitochondria-rich cells, zebrafish embryo
MeSH Terms
  • TRPV Cation Channels
  • Calcium/metabolism*
  • Calcium Signaling/physiology
  • Tissue Distribution
  • Zebrafish/embryology*
  • Zebrafish/metabolism*
  • Sequence Homology, Amino Acid
  • Calcium Channels/chemistry*
  • Calcium Channels/metabolism*
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/metabolism*
  • Gene Expression Regulation, Developmental/physiology*
  • Animals
  • Amino Acid Sequence
  • Molecular Sequence Data
  • Organ Specificity
(all 16)
PubMed
15947067 Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.
Abstract
The purpose of the present work was to study the possible role of the epithelial Ca(2+) channel (ECaC) in the Ca(2+) uptake mechanism in developing zebrafish (Danio rerio). With rapid amplification of cDNA ends, full-length cDNA encoding the ECaC of zebrafish (zECaC) was cloned and sequenced. The cloned zECaC was 2,578 bp in length and encoded a protein of 709 amino acids that showed up to 73% identity with previously described vertebrate ECaCs. The zECaC was found to be expressed in all tissues examined and began to be expressed in the skin covering the yolk sac of embryos at 24 h postfertilization (hpf). zECaC-expressing cells expanded to cover the skin of the entire yolk sac after embryonic development and began to occur in the gill filaments at 96 hpf, and thereafter zECaC-expressing cells rapidly increased in both gills and yolk sac skin. Corresponding to ECaC expression profile, the Ca(2+) influx and content began to increase at 36-72 hpf. Incubating zebrafish embryos in low-Ca(2+) (0.02 mM) freshwater caused upregulation of the whole body Ca(2+) influx and zECaC expression in both gills and skin. Colocalization of zECaC mRNA and the Na(+)-K(+)-ATPase alpha-subunit (a marker for mitochondria-rich cells) indicated that only a portion of the mitochondria-rich cells expressed zECaC mRNA. These results suggest that the zECaC plays a key role in Ca(2+) absorption in developing zebrafish.
Genes / Markers
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Expression
Phenotype
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Pan, T.C., Liao, B.K., Huang, C.J., Lin, L.Y., and Hwang, P.P. (2005) Epithelial Ca2+ Channel Expression and Ca2+ uptake in Developing Zebrafish. American journal of physiology. Regulatory, integrative and comparative physiology. 289(4):R1202-1211.