PUBLICATION
Sinup, a novel Siaz-interacting nuclear protein, modulates neural plate formation in the zebrafish embryos
- Authors
- Ro, H., Won, M., Lee, S.U., Kim, K.E., Huh, T.L., Kim, C.H., and Rhee, M.
- ID
- ZDB-PUB-050603-9
- Date
- 2005
- Source
- Biochemical and Biophysical Research Communications 332(4): 993-1003 (Journal)
- Registered Authors
- Huh, Tae-Lin, Kim, Cheol-Hee
- Keywords
- Siaz; Sinup; Yeast two-hybrid assay; Neural plate; Zebrafish
- MeSH Terms
-
- Signal Transduction
- Zebrafish Proteins/biosynthesis
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/metabolism*
- Zebrafish Proteins/physiology*
- Cell Nucleus/metabolism
- Blotting, Northern
- Gene Expression Regulation, Developmental*
- Humans
- Cytoskeletal Proteins/biosynthesis
- Protein Binding
- In Situ Hybridization
- Nuclear Proteins/chemistry
- Nuclear Proteins/metabolism*
- Two-Hybrid System Techniques
- Transfection
- Homeodomain Proteins/biosynthesis
- Animals
- Otx Transcription Factors
- Amino Acid Sequence
- Plasmids/metabolism
- DNA, Complementary/metabolism
- Ectoderm/metabolism
- Zebrafish
- Glutathione Transferase/metabolism
- Green Fluorescent Proteins/metabolism
- Wnt Proteins
- Base Sequence
- Neural Crest/embryology*
- Time Factors
- Oligonucleotides/chemistry
- Protein Structure, Tertiary
- Carrier Proteins/metabolism
- Carrier Proteins/physiology*
- Reverse Transcriptase Polymerase Chain Reaction
- Molecular Sequence Data
- RNA/chemistry
- Gene Library
- Ubiquitin-Protein Ligases
- PubMed
- 15922293 Full text @ Biochem. Biophys. Res. Commun.
Citation
Ro, H., Won, M., Lee, S.U., Kim, K.E., Huh, T.L., Kim, C.H., and Rhee, M. (2005) Sinup, a novel Siaz-interacting nuclear protein, modulates neural plate formation in the zebrafish embryos. Biochemical and Biophysical Research Communications. 332(4):993-1003.
Abstract
Siah, the vertebrate homologue of the Drosophila seven in absentia (sina) gene, is well conserved from Drosophila to mammal and involved in ubiquitination and proteasome-dependent degradation of various target proteins. To identify cellular proteins interacting with Siah, we screened a zebrafish cDNA library with zebrafish Siah (Siaz) as bait in a yeast two-hybrid assay. We identified a cDNA encoding a novel protein composed of 145 amino acids and termed it as Sinup (Siaz-interacting-nuclear-protein). Sinup is a novel nuclear protein that binds to the highly conserved C-terminal protein-interacting domain of Siaz both in vivo and in vitro. During development, sinup transcripts are abundant from the one-cell stage to the early blastula and then markedly diminished, suggesting sinup largely exists as maternal transcripts. sinup overexpression induced lateral expansion of the neural plate and in consequence caused ectopic expression of otx-2 and hoxb1b during the late gastrula stage. In addition, the lateral/paraxial expression of wnt8 at the onset of gastrulation is suppressed by the forced expression of sinup while the expression levels of various dorso-ventral markers are unaffected. In contrast, interfering with sinup functions using sinup morpholino oligonucleotides gradually diminished the anterior neuroectoderm from the posterior region, and resulted in compete loss of hindbrain at the 3-somites stage. Our report suggests that sinup expression should be tightly regulated during early embryonic development for the proper neural plate formation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping