PUBLICATION
Constitutive and xenobiotics-induced expression of a novel CYP3A gene from zebrafish larva
- Authors
- Tseng, H.P., Hseu, T.H., Buhler, D.R., Wang, W.D., and Hu, C.H.
- ID
- ZDB-PUB-050603-8
- Date
- 2005
- Source
- Toxicology and applied pharmacology 205(3): 247-258 (Journal)
- Registered Authors
- Buhler, Donald R., Hu, Chin-Hwa
- Keywords
- Xenobiotics; CYP3A; Zebrafish larva
- MeSH Terms
-
- Oligonucleotides, Antisense/pharmacology
- Sequence Analysis, Protein/methods
- Larva/drug effects
- Larva/genetics*
- Larva/metabolism
- Aryl Hydrocarbon Hydroxylases/drug effects
- Aryl Hydrocarbon Hydroxylases/genetics*
- Aryl Hydrocarbon Hydroxylases/metabolism
- Oxidoreductases, N-Demethylating/drug effects
- Oxidoreductases, N-Demethylating/genetics*
- Oxidoreductases, N-Demethylating/metabolism
- RNA, Messenger
- Signal Transduction/drug effects
- Signal Transduction/physiology
- Dexamethasone/pharmacology
- Receptors, Aryl Hydrocarbon/antagonists & inhibitors
- Receptors, Aryl Hydrocarbon/drug effects
- Rifampin/pharmacology
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/drug effects
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Animals
- Xenobiotics/chemistry
- Xenobiotics/pharmacology*
- Reverse Transcriptase Polymerase Chain Reaction/methods
- In Situ Hybridization/methods
- Gene Expression Regulation, Developmental/drug effects*
- Gene Expression Regulation, Developmental/genetics
- Intestines/drug effects
- Intestines/embryology
- PubMed
- 15922010 Full text @ Tox. App. Pharmacol.
- CTD
- 15922010
Citation
Tseng, H.P., Hseu, T.H., Buhler, D.R., Wang, W.D., and Hu, C.H. (2005) Constitutive and xenobiotics-induced expression of a novel CYP3A gene from zebrafish larva. Toxicology and applied pharmacology. 205(3):247-258.
Abstract
In mammals, CYP3A isozymes collectively comprise the largest portion of the liver and small intestinal CYP protein. They are involved in the metabolism of an extensive range of endogenous substrates and xenobiotics and make a significant contribution to the termination of the action of steroid hormones. A full-length cDNA of CYP3A gene, named CYP3A65, was cloned from zebrafish by RT-PCR. The CYP3A65 mRNA was initially transcribed only in the liver and intestine upon hatching of the zebrafish embryos. Like the human CYP3A genes, CYP3A65 transcription in the foregut region was enhanced by treatment of the zebrafish larvae with the steroid dexamethasone and the macrocyclic antibiotic rifampicin. Differing from mammalian CYP3A genes, CYP3A65 transcription was also elicited by 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD) during early larval stages. Repression of AHR2 translation by antisense morpholino oligonucleotides abrogated both of constitutive and TCDD-stimulated CYP3A65 transcription in larval intestine. These findings suggested that the AHR2 signaling pathway plays an essential role in CYP3A65 transcription.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping