PUBLICATION
MicroRNA Expression in Zebrafish Embryonic Development
- Authors
- Wienholds, E., Kloosterman, W.P., Miska, E., Alvarez-Saavedra, E., Berezikov, E., de Bruijn, E., Horvitz, R.H., Kauppinen, S., and Plasterk, R.H.
- ID
- ZDB-PUB-050603-3
- Date
- 2005
- Source
- Science (New York, N.Y.) 309(5732): 310-311 (Journal)
- Registered Authors
- Kloosterman, Wigard, Plasterk, Ronald H.A., Wienholds, Erno
- Keywords
- none
- Datasets
- GEO:GSE2628, GEO:GSE2625
- MeSH Terms
-
- Animals
- Blotting, Northern
- Embryo, Nonmammalian/metabolism*
- Embryonic Development
- Gene Expression*
- In Situ Hybridization
- MicroRNAs/genetics*
- MicroRNAs/metabolism*
- Multigene Family
- Oligonucleotide Array Sequence Analysis
- Oligonucleotide Probes
- Organ Specificity
- Time Factors
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish/metabolism
- PubMed
- 15919954 Full text @ Science
Citation
Wienholds, E., Kloosterman, W.P., Miska, E., Alvarez-Saavedra, E., Berezikov, E., de Bruijn, E., Horvitz, R.H., Kauppinen, S., and Plasterk, R.H. (2005) MicroRNA Expression in Zebrafish Embryonic Development. Science (New York, N.Y.). 309(5732):310-311.
Abstract
MicroRNAs (miRNAs) are small noncoding RNAs, approximately 21 nucleotides in length, that can regulate gene expression by base-pairing to partially complementary mRNAs. Regulation by miRNAs can play essential roles in embryonic development. We determined the temporal and spatial expression patterns of 115 conserved vertebrate miRNAs in zebrafish embryos by microarrays and by in situ hybridizations, using locked-nucleic-acid-modified oligonucleotide probes. Most miRNAs were expressed in a highly tissue-specific manner during segmentation and later stages, but not early in development, suggesting that their role is not in tissue fate establishment but in differentiation or maintenance of tissue identity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping