PUBLICATION
Developmental role of GnRH and PACAP in a zebrafish model
- Authors
- Sherwood, N.M., and Wu, S.
- ID
- ZDB-PUB-050506-8
- Date
- 2005
- Source
- General and comparative endocrinology 142(1-2): 74-80 (Review)
- Registered Authors
- Sherwood, Nancy M.
- Keywords
- GnRH; GHRH; PACAP; Neuropeptides; Development; Reproduction; Zebrafish; Fish; Morpholino; Brain; Gene knockdown
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Gonadotropin-Releasing Hormone/genetics
- Gonadotropin-Releasing Hormone/physiology*
- Nerve Growth Factors/genetics
- Nerve Growth Factors/physiology*
- Neuropeptides/genetics
- Neuropeptides/physiology*
- Neurotransmitter Agents/genetics
- Neurotransmitter Agents/physiology*
- Pituitary Adenylate Cyclase-Activating Polypeptide
- Receptors, Vasoactive Intestinal Peptide
- Zebrafish/genetics
- Zebrafish/growth & development*
- Zebrafish/physiology*
- Zebrafish Proteins
- PubMed
- 15862551 Full text @ Gen. Comp. Endocrinol.
Citation
Sherwood, N.M., and Wu, S. (2005) Developmental role of GnRH and PACAP in a zebrafish model. General and comparative endocrinology. 142(1-2):74-80.
Abstract
GnRH is expressed early in development long before reproduction begins. To determine whether GnRH has a role in development, gene knockdown with morpholinos was used in one-cell zebrafish embryos to block translation of gnrh mRNA into protein. Gene knockdown of gnrh2, gnrh3 or both at the one-cell stage resulted in a high percentage of embryos at 24-48h with a defective mid-hindbrain boundary and underdeveloped eyes; a small percentage of embryos at 72h had a defective heart. In similar studies on GHRH-PACAP, gene knockdown resulted in a smaller brain and eyes, but a normal-appearing heart. The evidence supports a role for the three neuropeptides in early development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping