PUBLICATION

Mutant-specific gene programs in the zebrafish

Authors
Weber, G.J., Choe, S.E., Dooley, K.A., Paffett-Lugassy, N.N., Zhou, Y., and Zon, L.I.
ID
ZDB-PUB-050419-2
Date
2005
Source
Blood   106(2): 521-530 (Journal)
Registered Authors
Dooley, Kim, Paffett-Lugassy, Noelle, Zhou, Yi, Zon, Leonard I.
Keywords
none
MeSH Terms
  • Animals
  • Blood Vessels/embryology
  • Erythropoiesis/genetics
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Hematopoiesis/genetics*
  • In Situ Hybridization
  • Multigene Family
  • Mutation*
  • Myelopoiesis/genetics
  • Phenotype
  • Transcription, Genetic
  • Zebrafish/embryology*
  • Zebrafish/genetics*
PubMed
15827125 Full text @ Blood
Abstract
Hematopoiesis involves the production of stem cells, followed by the orchestrated differentiation of the blood lineages. Genetic screens in zebrafish have identified mutants with defects that disrupt specific stages of hematopoiesis and vasculogenesis, including cloche, spadetail (tbx16), moonshine (tif1g), bloodless, and vlad tepes (gata1) mutants. To better characterize the blood program, gene expression profiling was carried out in these mutants and in scl-morphants (scl(mo)). Distinct gene clusters were demarcated by stage specific and mutant specific gene regulation. These were found to correlate with the transcriptional program of hematopoietic progenitor cells, as well as of the erythroid, myeloid, and vascular lineages. Among these, several novel hematopoietic and vascular genes were detected, for instance the erythroid transcription factors znfl2 and ncoa4. A specific regulation was found for myeloid genes as they were more strongly expressed in vlt mutants, compared to other erythroid mutants. A unique gene expression pattern of upregulated isoprenoid synthesis genes was found in cloche and scl(mo), possibly in migrating cells. In conjunction with the high conservation of vertebrate hematopoiesis, the comparison of transcriptional profiles in zebrafish blood mutants represents a versatile and powerful tool to elucidate the genetic regulation of blood and blood vessel development.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes