Two ribeye Genes in Teleosts: The Role of Ribeye in Ribbon Formation and Bipolar Cell Development
- Wan, L., Almers, W., and Chen, W.
- The Journal of neuroscience : the official journal of the Society for Neuroscience 25(4): 941-949 (Journal)
- Registered Authors
- Chen, Wenbiao
- Ribeye; optokinetic response; retina; synaptic ribbon; synaptogenesis; bipolar cells; apoptosis; morpholino antisense oligo
- MeSH Terms
- Eye Proteins/genetics
- Eye Proteins/physiology*
- Gene Expression Regulation, Developmental
- Larva/growth & development
- Oligodeoxyribonucleotides, Antisense
- Photic Stimulation
- Presynaptic Terminals/ultrastructure
- Retina/anatomy & histology*
- Retina/growth & development
- Takifugu/anatomy & histology*
- Takifugu/growth & development
- Zebrafish/anatomy & histology*
- Zebrafish/growth & development
- 15673675 Full text @ J. Neurosci.
Wan, L., Almers, W., and Chen, W. (2005) Two ribeye Genes in Teleosts: The Role of Ribeye in Ribbon Formation and Bipolar Cell Development. The Journal of neuroscience : the official journal of the Society for Neuroscience. 25(4):941-949.
Ribeye is the only known protein specific to synaptic ribbon, but its function is unclear. We show that the teleost fish, Fugu and zebrafish, have two ribeye genes, ribeye a and ribeye b. Whole-mount in situ hybridization revealed that ribeye a is expressed in tissues containing synaptic ribbons, including the pineal gland, inner ear, and retina. Ribeye b is absent in the pineal gland. In the retina, ribeye a is expressed in both photoreceptors and bipolar cells, whereas ribeye b is detected only in photoreceptors. To study the function of Ribeye a in retina, we depleted it by morpholino antisense oligos. Fish deficient in Ribeye a lack an optokinetic response and have shorter synaptic ribbons in photoreceptors and fewer synaptic ribbons in bipolar cells. Their bipolar cells still target Syntaxin-3 proteins to the inner plexiform layer and have abundant vsx1 mRNA. However, they lack large synaptic terminals and show increased apoptosis. Rod bipolar cells are fewer in number and/or deficient in PKCalpha. Recovery of Ribeye a levels rescues the optokinetic response, increases the number of PKCalpha-positive bipolar cells, and stops apoptosis. We conclude that Ribeye a is important for late steps in bipolar cell development.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes