PUBLICATION
A PKR-like eukaryotic initiation factor 2{alpha} kinase from zebrafish contains Z-DNA binding domains instead of dsRNA binding domains
- Authors
- Rothenburg, S., Deigendesch, N., Dittmar, K., Koch-Nolte, F., Haag, F., Lowenhaupt, K., and Rich, A.
- ID
- ZDB-PUB-050125-5
- Date
- 2005
- Source
- Proceedings of the National Academy of Sciences of the United States of America 102(5): 1602-1607 (Journal)
- Registered Authors
- Keywords
- E3L, interferon response, viral infection, Z-RNA, Z domain
- MeSH Terms
-
- Alternative Splicing
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Conserved Sequence
- DNA, Z-Form/metabolism*
- Humans
- Molecular Sequence Data
- Phylogeny
- Poly I-C/pharmacology
- Sequence Alignment
- Sequence Homology, Amino Acid
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- eIF-2 Kinase/genetics
- eIF-2 Kinase/metabolism*
- PubMed
- 15659550 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Rothenburg, S., Deigendesch, N., Dittmar, K., Koch-Nolte, F., Haag, F., Lowenhaupt, K., and Rich, A. (2005) A PKR-like eukaryotic initiation factor 2{alpha} kinase from zebrafish contains Z-DNA binding domains instead of dsRNA binding domains. Proceedings of the National Academy of Sciences of the United States of America. 102(5):1602-1607.
Abstract
The double-stranded RNA (dsRNA)-dependent protein kinase (PKR) is induced as part of the IFN response in mammals and acts to shut down protein synthesis by the phosphorylation of eukaryotic initiation factor 2alpha (eIF2alpha). In fish, a PKR-like kinase activity has been detected, but the enzyme responsible has eluded characterization. Here, we describe a PKR-like kinase from zebrafish. Phylogenetic analysis shows that the C-terminal kinase domain is more closely related to the kinase domain of PKR than to any of the other three known eIF2alpha kinases. Surprisingly, instead of the two dsRNA binding domains found at the N terminus of PKR, there are two Zalpha domains. Zalpha domains specifically bind dsDNA and RNA in the left-handed Z conformation, often with high affinity. They have been found previously in two other IFN-inducible proteins, the dsRNA editing enzyme, ADAR1, and Z-DNA binding protein 1 (ZBP1), as well as in the poxvirus virulence factor, E3L. This previously undescribed kinase, designated PKZ (protein kinase containing Z-DNA binding domains), is transcribed constitutively at low levels and is highly induced after injection of poly(inosinic)-poly(cytidylic) acid, which simulates viral infection. Binding of Z-DNA by the Zalpha domain of PKZ was demonstrated by circular dichroism. PKZ inhibits translation in transfected cells; site-directed mutagenesis indicates that this inhibition depends on its catalytic activity. Identification of a gene combining Zalpha domains with a PKR-like kinase domain strengthens the hypothesis that the ability to bind left-handed nucleic acid plays a role in the host response to viruses.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping