PUBLICATION

Using the zebrafish model to study GATA transcription factors

Authors
Heicklen-Klein, A., McReynolds, L.J., and Evans, T.
ID
ZDB-PUB-050125-3
Date
2005
Source
Seminars in cell & developmental biology   16(1): 95-106 (Journal)
Registered Authors
Evans, Todd
Keywords
Faust; Vlad tepes; Transgenic reporter fish; Hematopoiesis; Cardiogenesis
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • DNA-Binding Proteins/genetics*
  • Erythroid-Specific DNA-Binding Factors
  • GATA2 Transcription Factor
  • GATA3 Transcription Factor
  • GATA4 Transcription Factor
  • GATA5 Transcription Factor
  • GATA6 Transcription Factor
  • Heart/growth & development
  • Hematopoiesis
  • Models, Animal*
  • Molecular Sequence Data
  • Sequence Alignment
  • Trans-Activators/genetics
  • Transcription Factors/genetics*
  • Vertebrates/growth & development
  • Zebrafish/genetics*
  • Zebrafish Proteins
PubMed
15659344 Full text @ Sem. Cell Dev. Biol.
Abstract
The zebrafish is an established animal model system that profits from the availability of strong experimental approaches in both genetics and embryology. As a vertebrate, zebrafish can be used to model many aspects of human development and disease. GATA transcription factors play important roles in the development of many organ systems, including those for hematopoietic, cardiovascular, reproductive, and gut-endoderm derived tissues. The six vertebrate GATA factors are highly conserved in zebrafish at the level of sequence, expression pattern, and function. The identification of mutants, establishment of transgenic GFP reporter fish, and the ease of performing loss- and gain-of-function experiments have all contributed new insight into our understanding of the regulation and function of GATA factors. We review recent advances toward this goal using the zebrafish system with a focus on hematopoiesis and cardiogenesis, and suggest how comparative genetics using the zebrafish genes might reveal core conserved properties, as well as changes in gene function that reflect different morphogenetic programs utilized by various vertebrate embryos.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping