PUBLICATION
Expression of cyp26b1 during zebrafish early development
- Authors
- Zhao, Q., Dobbs-McAuliffe, B., and Linney, E.
- ID
- ZDB-PUB-050125-11
- Date
- 2005
- Source
- Gene expression patterns : GEP 5(3): 363-369 (Journal)
- Registered Authors
- Dobbs-McAuliffe, Betsy, Linney, Elwood, Zhao, Qingshun
- Keywords
- Zebrafish; Cyp26b1; Embryo; Development; Hindbrain; Rhombomere; Retinoid signaling; Pectoral fin; Retinoic acid; Cytochrome p450
- MeSH Terms
-
- Tretinoin/physiology
- Reverse Transcriptase Polymerase Chain Reaction
- Transcription, Genetic/drug effects
- Base Sequence
- Cloning, Molecular
- Cerebellum/enzymology
- Diencephalon/enzymology
- Amino Acid Sequence
- Rhombencephalon/enzymology
- Molecular Sequence Data
- In Situ Hybridization
- Cytochrome P-450 Enzyme System/biosynthesis*
- Cytochrome P-450 Enzyme System/chemistry
- Cytochrome P-450 Enzyme System/genetics
- Cytochrome P-450 Enzyme System/metabolism
- Animals
- Zebrafish Proteins/biosynthesis*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/growth & development*
- Zebrafish/metabolism
- Gene Expression
- PubMed
- 15661642 Full text @ Gene Expr. Patterns
Citation
Zhao, Q., Dobbs-McAuliffe, B., and Linney, E. (2005) Expression of cyp26b1 during zebrafish early development. Gene expression patterns : GEP. 5(3):363-369.
Abstract
We have cloned the zebrafish ortholog of the mammalian cyp26b1 gene. The predicted zebrafish cyp26b1 protein shares greater than 73% identity with mammalian homologues. cDNA transfection assays showed that like human cyp26b1, zebrafish cyp26b1 is involved in limiting the activity of retinoic acid. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of embryonic RNAs suggested that no maternal cyp26b1 message is detectable. Zygotic cyp26b1 message could be detected at 75% epiboly by RT-PCR and localized to presumptive rhombomere 3 and rhombomere 4 at the early two-somite (2S) stage (10.5 hpf: hour post fertilization) by whole mount in situ hybridization. As development proceeds expression expands anteriorly to include rhombomere 2 at the 10S stage (14hpf). By 14S (16hpf) expression in the hindbrain has also expanded posteriorly and encompasses rhombomere 2 through rhombomere 6. At later stages, 24 through 48 hpf, additional expression was found in the eyes, diencephalon, midbrain-hindbrain boundary, cerebellum, pectoral fin and the pharyngeal arch primordia.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping