ZFIN ID: ZDB-PUB-050119-8
Glycogen synthase kinase 3 has a proapoptotic function in Hydra gametogenesis
Rentzsch, F., Hobmayer, B., and Holstein, T.W.
Date: 2005
Source: Developmental Biology   278(1): 1-12 (Journal)
Registered Authors: Rentzsch, Fabian
Keywords: Cnidaria; Hydra; GSK3; Wnt; Oogenesis; Gametogenesis; Apoptosis; Nurse cell
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Apoptosis/physiology*
  • Conserved Sequence
  • Enzyme Inhibitors/pharmacology
  • Female
  • Gametogenesis/drug effects
  • Gametogenesis/physiology*
  • Glycogen Synthase Kinase 3/genetics
  • Glycogen Synthase Kinase 3/metabolism*
  • Hydra/cytology
  • Hydra/enzymology*
  • Hydra/genetics
  • Hydra/growth & development*
  • In Situ Hybridization
  • Lithium Chloride/pharmacology
  • Molecular Sequence Data
  • Oogenesis/drug effects
  • Sequence Homology, Amino Acid
  • Spermatogenesis
  • Zebrafish
PubMed: 15649456 Full text @ Dev. Biol.
In an approach to study the evolutionary conservation of molecules of the Wnt signal transduction pathway, we analyzed the function of the major negative regulator of this pathway, GSK3 (glycogen synthase kinase 3), in the basal metazoan Hydra. Microinjection assays reveal that HyGSK3 inhibits beta-catenin in zebrafish embryos, indicating that the function of GSK3 in the canonical Wnt signaling pathway is evolutionarily conserved. In Hydra, HyGSK3 transcripts are strongly upregulated during gametogenesis. Treatment of female polyps with the GSK3 inhibitors lithium and alsterpaullone prevents the differentiation of nurse cells and subsequent oocyte formation. Our data indicate that GSK3 is required for the early induction of apoptosis in germline cells, which has been shown to be an initial step in Hydra gametogenesis. Our experiments show that main functions of GSK3 are evolutionarily conserved and unique to multicellular animals, a conclusion which is additionally supported by the presence of specific regulatory domains in the HyGSK3 protein.