PUBLICATION

Formation of the retinotectal projection requires Esrom, an ortholog of PAM (protein associated with Myc)

Authors
D'Souza, J., Hendricks, M., Le Guyader, S., Subburaju, S., Grunewald, B., Scholich, K., and Jesuthasan, S.
ID
ZDB-PUB-041216-2
Date
2005
Source
Development (Cambridge, England)   132(2): 247-256 (Journal)
Registered Authors
Grunewald, Barbara, Jesuthasan, Suresh, Le Guyader, Sylvie, Subburaju, Sivan
Keywords
Retinotectal projection, Topographic mapping, Fasciculation, Axon branching, Visual system, Zebrafish
MeSH Terms
  • Zebrafish
  • Cloning, Molecular
  • Signal Transduction
  • Axons/physiology*
  • Chromosome Mapping
  • Microscopy, Fluorescence
  • Transgenes
  • Mixed Function Oxygenases/biosynthesis*
  • Mixed Function Oxygenases/genetics*
  • Mixed Function Oxygenases/physiology*
  • Adaptor Proteins, Signal Transducing/biosynthesis*
  • Adaptor Proteins, Signal Transducing/genetics*
  • Adaptor Proteins, Signal Transducing/physiology*
  • Animals
  • In Situ Hybridization
  • RNA/metabolism
  • Tumor Suppressor Proteins
  • Ubiquitin-Protein Ligases/metabolism
  • Genotype
  • Phosphorylation
  • Models, Biological
  • Zebrafish Proteins/biosynthesis*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/physiology
  • Ligands
  • Phenotype
  • Repressor Proteins/biosynthesis
  • Eye/embryology*
  • Retina/embryology*
  • Retina/metabolism
  • Models, Genetic
  • Growth Cones/metabolism*
  • Drosophila
(all 33)
PubMed
15590740 Full text @ Development
Abstract
Visual system development is dependent on correct interpretation of cues that direct growth cone migration and axon branching. Mutations in the zebrafish esrom gene disrupt bundling and targeting of retinal axons, and also cause ectopic arborization. By positional cloning, we establish that esrom encodes a very large protein orthologous to PAM (protein associated with Myc)/Highwire/RPM-1. Unlike motoneurons in Drosophila highwire mutants, retinal axons in esrom mutants do not arborize excessively, indicating that Esrom has different functions in the vertebrate visual system. We show here that Esrom has E3 ligase activity and modulates the amount of phosphorylated Tuberin, a tumor suppressor, in growth cones. These data identify a mediator of signal transduction in retinal growth cones, which is required for topographic map formation.
Genes / Markers
Figures
Figure Gallery (7 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
te250
    		Small Deletion
    te275
      		Insertion
      tn207b
        		Point Mutation
        tp203
          		Point Mutation
          1 - 4 of 4
          Show
          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          mycbp2MO1-mycbp2MRPHLNO
          mycbp2MO2-mycbp2MRPHLNO
          1 - 2 of 2
          Show
          Fish
          Fish
          mycbp2te250/te250
          mycbp2te275/te275
          mycbp2tn207b/tn207b
          mycbp2tp203/tp203
          WT
          1 - 5 of 5
          Show
          Antibodies
          No data available
          Orthology
          Gene Orthology
          mycbp2
          1 - 1 of 1
          Show
          Engineered Foreign Genes
          No data available
          Mapping
          Entity Type Entity Symbol Location
          ESTfa97c06Chr: 9 Details
          Featurete250Ambiguous Details
          SSLPz6663Chr: 9 Details
          SSLPz7813Chr: 9 Details
          1 - 4 of 4
          Show
          Your Input Welcome
          We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
          Citation
          D'Souza, J., Hendricks, M., Le Guyader, S., Subburaju, S., Grunewald, B., Scholich, K., and Jesuthasan, S. (2005) Formation of the retinotectal projection requires Esrom, an ortholog of PAM (protein associated with Myc). Development (Cambridge, England). 132(2):247-256.