ZFIN ID: ZDB-PUB-041216-1
Zebrafish (Danio rerio) matrilins: shared and divergent characteristics with their mammalian counterparts
Ko, Y.P., Kobbe, B., Paulsson, M., and Wagener, R.
Date: 2005
Source: The Biochemical journal   386(Pt 2): 367-79 (Journal)
Registered Authors:
Keywords: Danio rerio (zebrafish), extracellular matrix, matrilin, von Willebrand factor A-like.
MeSH Terms:
  • Alternative Splicing/genetics
  • Animals
  • Cartilage Oligomeric Matrix Protein
  • Cell Line
  • Cloning, Molecular/methods
  • Conserved Sequence/genetics
  • Exons/genetics
  • Extracellular Matrix Proteins/genetics*
  • Extracellular Matrix Proteins/immunology
  • Gene Expression Regulation/genetics
  • Gene Expression Regulation, Developmental/genetics
  • Genetic Variation/genetics*
  • Glycoproteins/genetics*
  • Glycoproteins/immunology
  • Humans
  • Immune Sera/metabolism
  • Introns/genetics
  • Kidney/chemistry
  • Kidney/cytology
  • Kidney/embryology
  • Kidney/metabolism
  • Matrilin Proteins
  • Molecular Sequence Data
  • Peptides/genetics
  • Peptides/immunology
  • Radiation Hybrid Mapping/methods
  • Recombinant Proteins/genetics
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/immunology
PubMed: 15588228 Full text @ Biochem. J.
We have cloned the cDNAs of the zebrafish members of the matrilin family of extracellular adaptor proteins. In contrast to in mammals no ortholog of matrilin-2 was found in zebrafish, either by RT-PCR using degenerated primers or by screeening the databases, however two forms of matrilin-3, matrilin-3a and -3b, were present. The identity to the mammalian matrilins ranges from more than 70% for the VWA domains to only 28% for the coiled-coil domains of matrilin-3a and -3b. In all zebrafish matrilins we found a greater variety of splice variants than in mammals, with splicing mainly affecting the number of EGF-like repeats. The exon/intron organisation is nearly identical to that of mammals and also the characteristic AT-AC intron interupting the exons coding for the coiled-coil domain is conserved. In the matrilin-3b gene a unique exon codes for a proline and serine/threonine-rich domain, possibly having mucin-like properties. The matrilin-1 and -3a genes were mapped to chomosome 19 and 20, respectively, by the radiation hybrid method. The temporal and spatial expression of zebrafish matrilins is similar to that seen in the mouse. Zebrafish matrilin-4 is highly expressed as early as 24 hpf, whereas the other matrilins show peak expression at 72 hpf. By immunostaining of whole mounts and sections we found that matrilin-1 and -3a show predominantly a skeletal staining, whereas matrilin-4 is more widespread with the protein also being present in loose connective tissues and epithelia.