PUBLICATION
Expression of PKC in the developing zebrafish, Danio rerio
- Authors
- Blakey Slatter, C.A., Kanji, H., Coutts, C.A., and Ali, D.W.
- ID
- ZDB-PUB-041122-5
- Date
- 2005
- Source
- Journal of neurobiology 62(4): 425-438 (Journal)
- Registered Authors
- Keywords
- Rohon-Beard, apoptosis, trigeminal ganglia, dorsal root ganglion, Mauthner cell, PKCalpha, PKC alpha
- MeSH Terms
-
- Animals
- Gene Expression Regulation, Developmental/physiology*
- Immunohistochemistry
- Larva/enzymology
- Larva/growth & development
- Protein Kinase C/biosynthesis*
- Protein Kinase C-alpha
- Zebrafish/embryology*
- Zebrafish/metabolism*
- PubMed
- 15547934 Full text @ J. Neurobiol.
Citation
Blakey Slatter, C.A., Kanji, H., Coutts, C.A., and Ali, D.W. (2005) Expression of PKC in the developing zebrafish, Danio rerio. Journal of neurobiology. 62(4):425-438.
Abstract
Protein kinase C (PKC) is a family of enzymes involved in a wide range of biological functions. We investigated the expression of PKC-positive cells in zebrafish embryos and larvae within the first week of development to determine the developmental profile of PKC-containing cells. Our other goal was to determine if PKCalpha was associated with Rohon-Beard neurons during the first 5 days of development, when they are reported to undergo apoptosis. First, we confirmed the specificity of the antibodies by Western blotting zebrafish brain homogenates with anti-PKC and anti-PKCalpha, and detected single protein bands of approximately 78-82 kDa in size. Immunohistochemistry showed that several types of neurons were labeled, including neurons in the trigeminal ganglia, the dorsal spinal cord, and the dorsal root ganglia. Double-labeling with anti-PKCalpha and both anti-Islet-1 and zn12 confirmed the identity of the PKC-positive cells in the brain as trigeminal neurons, and in the spinal cord as Rohon-Beard cells. Some Rohon-Beard cells were labeled with anti-PKCalpha up to 7 days post fertilization (dpf). We performed TUNEL labeling and found no correlation between TUNEL-labeled and PKCalpha-labeled Rohon-Beard cells, suggesting that PKCalpha is not involved in Rohon-Beard apoptosis. Only approximately 40% of the approximately 130 Rohon-Beard cells at 24 h postfertilization (hpf) were positively labeled for PKC. Mauthner cells were labeled by anti-PKC, but not anti-PKCalpha, suggesting that the major form of PKC within these cells was not PKCalpha. (c) 2004 Wiley Periodicals, Inc. J Neurobiol, 2005.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping