PUBLICATION
Identification of a novel splicing form of zebrafish p73 having a strong transcriptional activity
- Authors
- Satoh, S., Arai, K., and Watanabe, S.
- ID
- ZDB-PUB-041116-1
- Date
- 2004
- Source
- Biochemical and Biophysical Research Communications 325(3): 835-842 (Journal)
- Registered Authors
- Watanabe, Sumiko
- Keywords
- p73; Zebrafish; Alternative splicing; Transcriptional activity
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cell Line
- Gene Expression Regulation/physiology*
- Humans
- Molecular Sequence Data
- Organ Specificity
- Osteoblasts/metabolism*
- Protein Isoforms/genetics
- Protein Isoforms/metabolism
- Protein Splicing/genetics
- Recombinant Proteins/genetics
- Recombinant Proteins/metabolism
- Sequence Homology, Amino Acid
- Tissue Distribution
- Transcription Factors/chemistry*
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Transcriptional Activation/physiology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/chemistry*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 15541366 Full text @ Biochem. Biophys. Res. Commun.
Citation
Satoh, S., Arai, K., and Watanabe, S. (2004) Identification of a novel splicing form of zebrafish p73 having a strong transcriptional activity. Biochemical and Biophysical Research Communications. 325(3):835-842.
Abstract
p73 is a transcriptional activator related to tumor suppressor p53 and regulates differentiation, cell-cycle arrest, and apoptosis. Recently, zebrafish p73 (zp73alpha) was isolated and shown to be required for zebrafish embryogenesis. In this study, we isolated a novel splicing-variant of zp73 mRNA, which was generated by the use of an alternative splicing acceptor site, and designated it as zp73theta;. The zp73theta; mRNA encoded a carboxy-terminal structure distinct from that of zp73alpha. Whereas the expression level of zp73theta; mRNA was much lower than that of zp73alpha in zebrafish adult tissues, it was relatively high and fluctuated during embryogenesis. Using Saos-2 cells for a transient reporter assay, we found that zp73theta;, but not zp73alpha, had strong transcriptional activity when the experiments were performed at 34 degrees C. In addition, zp73theta; had the ability to suppress the growth of Saos-2 cells and to cause the developmental defects in zebrafish. These data indicated that zp73theta; could work as a transcriptional activator in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping