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ZFIN ID: ZDB-PUB-041116-1
Identification of a novel splicing form of zebrafish p73 having a strong transcriptional activity
Satoh, S., Arai, K., and Watanabe, S.
Date: 2004
Source: Biochemical and Biophysical Research Communications   325(3): 835-842 (Journal)
Registered Authors: Watanabe, Sumiko
Keywords: p73; Zebrafish; Alternative splicing; Transcriptional activity
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Gene Expression Regulation/physiology*
  • Humans
  • Molecular Sequence Data
  • Organ Specificity
  • Osteoblasts/metabolism*
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • Protein Splicing/genetics
  • Recombinant Proteins/genetics
  • Recombinant Proteins/metabolism
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Transcription Factors/chemistry*
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Transcriptional Activation/physiology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/chemistry*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 15541366 Full text @ Biochem. Biophys. Res. Commun.
p73 is a transcriptional activator related to tumor suppressor p53 and regulates differentiation, cell-cycle arrest, and apoptosis. Recently, zebrafish p73 (zp73alpha) was isolated and shown to be required for zebrafish embryogenesis. In this study, we isolated a novel splicing-variant of zp73 mRNA, which was generated by the use of an alternative splicing acceptor site, and designated it as zp73theta;. The zp73theta; mRNA encoded a carboxy-terminal structure distinct from that of zp73alpha. Whereas the expression level of zp73theta; mRNA was much lower than that of zp73alpha in zebrafish adult tissues, it was relatively high and fluctuated during embryogenesis. Using Saos-2 cells for a transient reporter assay, we found that zp73theta;, but not zp73alpha, had strong transcriptional activity when the experiments were performed at 34 degrees C. In addition, zp73theta; had the ability to suppress the growth of Saos-2 cells and to cause the developmental defects in zebrafish. These data indicated that zp73theta; could work as a transcriptional activator in zebrafish.