PUBLICATION
Cloning and expression pattern of vat-1 homolog gene in zebrafish
- Authors
- Loeb-Hennard, C., Cousin, X., Prengel, I., and Kremmer, E.
- ID
- ZDB-PUB-041111-20
- Date
- 2004
- Source
- Gene expression patterns : GEP 5(1): 91-96 (Journal)
- Registered Authors
- Cousin, Xavier
- Keywords
- Zebrafish; Danio rerio; VAT-1; Brain; Neuronal clusters; Hindbrain; Neural tube; Gut; Pharynx
- MeSH Terms
-
- Adenosine Triphosphatases/biosynthesis
- Adenosine Triphosphatases/genetics*
- Amino Acid Sequence
- Animals
- Base Sequence
- Brain/embryology
- Brain/metabolism
- Branchial Region/embryology
- Branchial Region/metabolism
- Gene Expression Profiling
- Humans
- Mice
- Molecular Sequence Data
- Retina/embryology
- Retina/metabolism
- Sequence Alignment
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/biosynthesis
- Zebrafish Proteins/genetics*
- PubMed
- 15533823 Full text @ Gene Expr. Patterns
Citation
Loeb-Hennard, C., Cousin, X., Prengel, I., and Kremmer, E. (2004) Cloning and expression pattern of vat-1 homolog gene in zebrafish. Gene expression patterns : GEP. 5(1):91-96.
Abstract
The VAT-1 protein is present in the electric organ of marine rays where it is suggested to play a central role in nerve signal transmission. VAT-1 homolog protein was also identified in mouse and human but its function remains to be determined. We have investigated VAT-1 homolog in zebrafish Danio rerio since it is an excellent model amenable to the combination of genetic, molecular and embryological studies. Amino acid sequence analysis shows that the zebrafish VAT-1 homolog shares approximately 51-61% identity with the electric ray, mouse, and human counterparts. By in situ hybridization, vat-1 homolog mRNA is first observed in the trigeminal nuclei at the 8-somite stage. At 20-somite stage, vat-1 homolog is detected in the brain, namely in primary clusters of neurons, in the epiphysis and in the hindbrain. vat-1 homolog is also present in the neural tube but this expression disappears after 72 h post-fertilization. At 24 h post-fertilization, vat-1 homolog starts to be expressed in the developing gut. At later stages, vat-1 homolog is present throughout the brain, appears in the maturing retina and the pharyngeal cavity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping