PUBLICATION
Prox1 is a novel coregulator of Ff1b and is involved in the embryonic development of the zebra fish interrenal primordium
- Authors
- Liu, Y.W., Gao, W., Teh, H.L., Tan, J.H., and Chan, W.K.
- ID
- ZDB-PUB-041109-1
- Date
- 2003
- Source
- Molecular and cellular biology 23(20): 7243-7255 (Journal)
- Registered Authors
- Chan, Woon-Khiong, Liu, Yi-wen
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Blotting, Western
- DNA-Binding Proteins/metabolism*
- Dose-Response Relationship, Drug
- Down-Regulation
- Electrophoresis, Polyacrylamide Gel
- Fushi Tarazu Transcription Factors
- Homeodomain Proteins/metabolism
- Homeodomain Proteins/physiology*
- In Situ Hybridization
- Kidney/embryology*
- Liver/metabolism
- Models, Genetic
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Plasmids/metabolism
- Precipitin Tests
- Protein Binding
- Protein Isoforms
- Protein Structure, Tertiary
- Receptors, Cytoplasmic and Nuclear
- Steroidogenic Factor 1
- Transcription Factors/metabolism*
- Transcription, Genetic
- Tumor Suppressor Proteins
- Two-Hybrid System Techniques
- Zebrafish
- Zebrafish Proteins/metabolism*
- beta-Galactosidase/metabolism
- PubMed
- 14517294 Full text @ Mol. Cell. Biol.
Citation
Liu, Y.W., Gao, W., Teh, H.L., Tan, J.H., and Chan, W.K. (2003) Prox1 is a novel coregulator of Ff1b and is involved in the embryonic development of the zebra fish interrenal primordium. Molecular and cellular biology. 23(20):7243-7255.
Abstract
Steroidogenic factor 1 (SF-1) plays an essential role in adrenal development, although the exact molecular mechanisms are unclear. Our previous work established that Ff1b is the zebra fish homologue of SF-1 and that its disruption by antisense morpholinos leads to a complete ablation of the interrenal organ. In this study, results of biochemical analyses suggest that Ff1b and other Ff1 members interact with Prox1, a homeodomain protein. Fine mapping using site-directed mutants showed that this interaction requires an intact Ff1b heptad 9 and AF2, as well as Prox1 NR Box I. In vivo, this physical interaction led to the inhibition of Ff1-mediated transactivation of pLuc3XFRE, indicating that Prox1 acts to repress the transcriptional activity of Ff1b. In situ hybridization demonstrates that prox1 colocalizes with ff1a and ff1b in the liver and interrenal primordia, respectively. Embryos microinjected with prox1 morpholino displayed a consistent partial reduction of 3 eta-Hsd activity in the interrenal organ, while ff1b morpholino led to a disappearance of prox1. Based on these results, we propose that during the course of interrenal organogenesis, Prox1 functions as a tissue-specific coregulator of Ff1b and that the subsequent inhibition of Ff1b activity, after its initial roles in the specification of interrenal primordium, is critical for the maturation of the interrenal organ.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping