PUBLICATION

Twisted gastrulation promotes BMP signaling in zebrafish dorsal-ventral axial patterning

Authors
Little, S.C., and Mullins, M.C.
ID
ZDB-PUB-041108-1
Date
2004
Source
Development (Cambridge, England)   131(23): 5825-5835 (Journal)
Registered Authors
Little, Shawn, Mullins, Mary C.
Keywords
Twisted gastrulation, BMP signaling, Bone morphogenetic protein, Chordin, Zebrafish
MeSH Terms
  • Animals
  • RNA/metabolism
  • Phenotype
  • Genotype
  • Heterozygote
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/physiology*
  • Glycoproteins/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger/metabolism
  • Cell Lineage
  • Blotting, Western
  • Signal Transduction*
  • Gastrula/metabolism*
  • Zebrafish
  • Transgenes
  • Time Factors
  • Intercellular Signaling Peptides and Proteins/metabolism
  • In Situ Hybridization
  • Bone Morphogenetic Proteins/metabolism*
  • Drosophila Proteins/genetics
(all 21)
PubMed
15525664 Full text @ Development
Abstract
In vertebrates and invertebrates, the bone morphogenetic protein (BMP) signaling pathway patterns cell fates along the dorsoventral (DV) axis. In vertebrates, BMP signaling specifies ventral cell fates, whereas restriction of BMP signaling by extracellular antagonists allows specification of dorsal fates. In misexpression assays, the conserved extracellular factor Twisted gastrulation (Tsg) is reported to both promote and antagonize BMP signaling in DV patterning. To investigate the role of endogenous Tsg in early DV patterning, we performed morpholino (MO)-based knockdown studies of Tsg1 in zebrafish. We found that loss of tsg1 results in a moderately strong dorsalization of the embryonic axis, suggesting that Tsg1 promotes ventral fates. Knockdown of tsg1 combined with loss of function of the BMP agonist tolloid (mini fin) or heterozygosity for the ligand bmp2b (swirl) enhanced dorsalization, supporting a role for Tsg1 in specifying ventral cell fates as a BMP signaling agonist. Moreover, loss of tsg1 partially suppressed the ventralized phenotypes of mutants of the BMP antagonists Chordin or Sizzled (Ogon). Our results support a model in which zebrafish Tsg1 promotes BMP signaling, and thus ventral cell fates, during DV axial patterning.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
tc300a
    		Point Mutation
    tm124a
      		Point Mutation
      tm305
        		Point Mutation
        tt250
          		Point Mutation
          1 - 4 of 4
          Show
          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          twsg1bMO1-twsg1bMRPHLNO
          twsg1bMO2-twsg1bMRPHLNO
          twsg1bMO3-twsg1bMRPHLNO
          1 - 3 of 3
          Show
          Fish
          Fish
          bmp2btc300a/tc300a + MO1-twsg1b (TU)
          bmp2btc300a/tc300a (TU)
          chrdtt250/tt250
          chrdtt250/tt250 + MO3-twsg1b
          szltm305/tm305
          szltm305/tm305 + MO1-twsg1b
          tll1tm124a/tm124a (TU)
          WT
          WT + MO1-twsg1b
          WT + MO3-twsg1b
          1 - 10 of 10
          Show
          Antibodies
          No data available
          Orthology
          No data available
          Engineered Foreign Genes
          No data available
          Mapping
          No data available
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          Citation
          Little, S.C., and Mullins, M.C. (2004) Twisted gastrulation promotes BMP signaling in zebrafish dorsal-ventral axial patterning. Development (Cambridge, England). 131(23):5825-5835.