PUBLICATION
Plakoglobin expression and localization in zebrafish embryo development
- Authors
- Martin, E.D., and Grealy, M.
- ID
- ZDB-PUB-041022-3
- Date
- 2004
- Source
- Biochemical Society transactions 32(Pt 5): 797-8 (Journal)
- Registered Authors
- Keywords
- b-catenin, desmosomes, heart, plakoglobin, zebrafish
- MeSH Terms
-
- Adherens Junctions/metabolism*
- Animals
- Blotting, Western
- Cell Adhesion
- Cytoskeletal Proteins/biosynthesis*
- Cytoskeletal Proteins/metabolism
- Cytoskeletal Proteins/physiology*
- Desmoplakins
- Desmosomes/metabolism*
- Embryo, Nonmammalian/physiology*
- Gene Expression Regulation, Developmental*
- Heart/embryology*
- Microscopy, Confocal
- Time Factors
- Trans-Activators/metabolism
- Zebrafish
- Zebrafish Proteins
- beta Catenin
- gamma Catenin
- PubMed
- 15494018 Full text @ Biochem Soc. Trans.
Citation
Martin, E.D., and Grealy, M. (2004) Plakoglobin expression and localization in zebrafish embryo development. Biochemical Society transactions. 32(Pt 5):797-8.
Abstract
Plakoglobin (gamma-catenin) and beta-catenin are major components of the adherens junctions and can be localized to the nucleus by activation of the Wnt signalling pathway. In addition, plakoglobin is also found in desmosomes, a vertebrate-specific cell-cell adhesion structure. Plakoglobin expression and localization were examined at the protein level during zebrafish embryonic development by Western blotting and confocal microscopy. Plakoglobin was expressed throughout embryo development at the protein level. Western blotting revealed that embryonic plakoglobin protein content increased between 12- and 24-h post-fertilization (hpf). Confocal microscopy showed that at stages up to 12 hpf, plakoglobin and beta-catenin were co-localized and expressed in both the nucleus and in cell-cell junctions. At 24- and 72-hpf, separate patterns were seen for plakoglobin and beta-catenin. These data indicate that plakoglobin localization in the heart region shifts from adherens junctions to desmosomes during heart chamber development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping