PUBLICATION
TRPA1 is a candidate for the mechanosensitive transduction channel of vertebrate hair cells
- Authors
- Corey, D.P., García-Añoveros, J., Holt, J.R., Kwan, K.Y., Lin, S.Y., Vollrath, M.A., Amalfitano, A., Cheung, E.L., Derfler, B.H., Duggan, A., Géléoc, G.S., Gray, P.A., Hoffman, M.P., Rehm, H.L., Tamasauskas, D., Zhang, D.S.
- ID
- ZDB-PUB-041018-3
- Date
- 2004
- Source
- Nature 432(7018): 723-730 (Journal)
- Registered Authors
- Lin, Shuh-Yow
- Keywords
- none
- MeSH Terms
-
- Transient Receptor Potential Channels
- Antibodies/immunology
- RNA, Small Interfering/genetics
- RNA, Small Interfering/metabolism
- Mechanotransduction, Cellular/physiology*
- Vertebrates/metabolism*
- Hearing/physiology*
- Ear, Inner/metabolism
- In Situ Hybridization
- Mice
- Animals
- Rana catesbeiana
- Zebrafish Proteins/biosynthesis
- Zebrafish Proteins/genetics
- Zebrafish Proteins/immunology
- Zebrafish Proteins/metabolism*
- Ion Channels/biosynthesis
- Ion Channels/genetics
- Ion Channels/immunology
- Ion Channels/metabolism*
- Zebrafish/metabolism
- Animals, Newborn
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Adenoviridae/genetics
- Oligonucleotides/genetics
- Oligonucleotides/metabolism
- Gene Expression Regulation, Developmental
- Hair Cells, Auditory/metabolism*
- PubMed
- 15483558 Full text @ Nature
Citation
Corey, D.P., García-Añoveros, J., Holt, J.R., Kwan, K.Y., Lin, S.Y., Vollrath, M.A., Amalfitano, A., Cheung, E.L., Derfler, B.H., Duggan, A., Géléoc, G.S., Gray, P.A., Hoffman, M.P., Rehm, H.L., Tamasauskas, D., Zhang, D.S. (2004) TRPA1 is a candidate for the mechanosensitive transduction channel of vertebrate hair cells. Nature. 432(7018):723-730.
Abstract
Mechanical deflection of the sensory hair bundles of receptor cells in the inner ear causes ion channels located at the tips of the bundle to open, thereby initiating the perception of sound. Although some protein constituents of the transduction apparatus are known, the mechanically gated transduction channels have not been identified in higher vertebrates. Here, we investigate TRP (transient receptor potential) ion channels as candidates and find one, TRPA1 (also known as ANKTM1), that meets criteria for the transduction channel. The appearance of TRPA1 messenger RNA expression in hair cell epithelia coincides developmentally with the onset of mechanosensitivity. Antibodies to TRPA1 label hair bundles, especially at their tips, and tip labelling disappears when the transduction apparatus is chemically disrupted. Inhibition of TRPA1 protein expression in zebrafish and mouse inner ears inhibits receptor cell function, as assessed with electrical recording and with accumulation of a channel-permeant fluorescent dye. TRPA1 is probably a component of the transduction channel itself.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping