PUBLICATION
Inhibition of Wnt signaling by ICAT, a novel beta-catenin-interacting protein
- Authors
- Tago, K., Nakamura, T., Nishita, M., Hyodo, J., Nagai, S., Murata, Y., Adachi, S., Ohwada, S., Morishita, Y., Shibuya, H., and Akiyama, T.
- ID
- ZDB-PUB-041006-12
- Date
- 2000
- Source
- Genes & Development 14(14): 1741-1749 (Journal)
- Registered Authors
- Keywords
- Wnt; beta-catenin; TCF; ICAT; signaling
- MeSH Terms
-
- Luciferases/metabolism
- Dose-Response Relationship, Drug
- Homeodomain Proteins/metabolism
- Transcription Factors/metabolism
- Body Patterning/drug effects
- Transfection
- Cell Cycle Proteins*
- Proto-Oncogene Proteins/antagonists & inhibitors*
- beta Catenin
- TCF Transcription Factors
- Mice
- Muscle Proteins/genetics
- Muscle Proteins/metabolism*
- Muscle Proteins/physiology
- Mutagenesis
- Genes, Dominant
- Repressor Proteins*
- Immunoblotting
- Precipitin Tests
- Xenopus/embryology
- Humans
- Xenopus Proteins
- Gene Expression Regulation, Developmental/drug effects
- Tumor Cells, Cultured
- Signal Transduction*
- Intracellular Signaling Peptides and Proteins
- Goosecoid Protein
- Transcription Factor 7-Like 2 Protein
- Two-Hybrid System Techniques
- Transcription, Genetic
- Trans-Activators*
- Amino Acid Sequence
- Animals
- Molecular Sequence Data
- Wnt Proteins
- Cytoskeletal Proteins/genetics
- Cytoskeletal Proteins/metabolism*
- Cytoskeletal Proteins/physiology
- Cell Division
- Zebrafish Proteins*
- Gene Library
- PubMed
- 10898789 Full text @ Genes & Dev.
Citation
Tago, K., Nakamura, T., Nishita, M., Hyodo, J., Nagai, S., Murata, Y., Adachi, S., Ohwada, S., Morishita, Y., Shibuya, H., and Akiyama, T. (2000) Inhibition of Wnt signaling by ICAT, a novel beta-catenin-interacting protein. Genes & Development. 14(14):1741-1749.
Abstract
Wnt signaling has an important role in both embryonic development and tumorigenesis. beta-Catenin, a key component of the Wnt signaling pathway, interacts with the TCF/LEF family of transcription factors and activates transcription of Wnt target genes. Here, we identify a novel beta-catenin-interacting protein, ICAT, that was found to inhibit the interaction of beta-catenin with TCF-4 and represses beta-catenin-TCF-4-mediated transactivation. Furthermore, ICAT inhibited Xenopus axis formation by interfering with Wnt signaling. These results suggest that ICAT negatively regulates Wnt signaling via inhibition of the interaction between beta-catenin and TCF and is integral in development and cell proliferation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping