PUBLICATION
Inhibition of Wnt signaling by ICAT, a novel beta-catenin-interacting protein
- Authors
- Tago, K., Nakamura, T., Nishita, M., Hyodo, J., Nagai, S., Murata, Y., Adachi, S., Ohwada, S., Morishita, Y., Shibuya, H., and Akiyama, T.
- ID
- ZDB-PUB-041006-12
- Date
- 2000
- Source
- Genes & Development 14(14): 1741-1749 (Journal)
- Registered Authors
- Keywords
- Wnt; beta-catenin; TCF; ICAT; signaling
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Body Patterning/drug effects
- Cell Cycle Proteins*
- Cell Division
- Cytoskeletal Proteins/genetics
- Cytoskeletal Proteins/metabolism*
- Cytoskeletal Proteins/physiology
- Dose-Response Relationship, Drug
- Gene Expression Regulation, Developmental/drug effects
- Gene Library
- Genes, Dominant
- Goosecoid Protein
- Homeodomain Proteins/metabolism
- Humans
- Immunoblotting
- Intracellular Signaling Peptides and Proteins
- Luciferases/metabolism
- Mice
- Molecular Sequence Data
- Muscle Proteins/genetics
- Muscle Proteins/metabolism*
- Muscle Proteins/physiology
- Mutagenesis
- Precipitin Tests
- Proto-Oncogene Proteins/antagonists & inhibitors*
- Repressor Proteins*
- Signal Transduction*
- TCF Transcription Factors
- Trans-Activators*
- Transcription Factor 7-Like 2 Protein
- Transcription Factors/metabolism
- Transcription, Genetic
- Transfection
- Tumor Cells, Cultured
- Two-Hybrid System Techniques
- Wnt Proteins
- Xenopus/embryology
- Xenopus Proteins
- Zebrafish Proteins*
- beta Catenin
- PubMed
- 10898789 Full text @ Genes & Dev.
Citation
Tago, K., Nakamura, T., Nishita, M., Hyodo, J., Nagai, S., Murata, Y., Adachi, S., Ohwada, S., Morishita, Y., Shibuya, H., and Akiyama, T. (2000) Inhibition of Wnt signaling by ICAT, a novel beta-catenin-interacting protein. Genes & Development. 14(14):1741-1749.
Abstract
Wnt signaling has an important role in both embryonic development and tumorigenesis. beta-Catenin, a key component of the Wnt signaling pathway, interacts with the TCF/LEF family of transcription factors and activates transcription of Wnt target genes. Here, we identify a novel beta-catenin-interacting protein, ICAT, that was found to inhibit the interaction of beta-catenin with TCF-4 and represses beta-catenin-TCF-4-mediated transactivation. Furthermore, ICAT inhibited Xenopus axis formation by interfering with Wnt signaling. These results suggest that ICAT negatively regulates Wnt signaling via inhibition of the interaction between beta-catenin and TCF and is integral in development and cell proliferation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping