ZFIN ID: ZDB-PUB-040920-6
Histone deacetylase 1 (HDAC-1) required for the normal formation of craniofacial cartilage and pectoral fins of the zebrafish
Pillai, R., Coverdale, L.E., Dubey, G., and Martin, C.C.
Date: 2004
Source: Developmental dynamics : an official publication of the American Association of Anatomists   231(3): 647-654 (Journal)
Registered Authors: Martin, C. Cristofre
Keywords: histone deacetylase, cartilage, fin bud, heart, morpholino, insertional mutant, zebrafish
MeSH Terms:
  • Alcian Blue/metabolism
  • Animals
  • Cartilage/embryology*
  • Embryo, Mammalian/drug effects
  • Embryo, Nonmammalian
  • Embryonic Development
  • Extremities/embryology*
  • Gene Expression Regulation, Developmental
  • Histone Deacetylase 1
  • Histone Deacetylases/metabolism
  • Histone Deacetylases/physiology*
  • Homozygote
  • In Situ Hybridization
  • Mutagenesis, Insertional
  • Oligonucleotides, Antisense/pharmacology
  • Skull/embryology*
  • Time Factors
  • Zebrafish/embryology*
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology*
PubMed: 15376317 Full text @ Dev. Dyn.
Histone deacetylases interact with nucleosomes to facilitate the formation of transcriptionally repressed chromatin. In the present study, we show that histone deacetylase 1 (hdac-1) is expressed throughout embryonic development of the zebrafish. The expression of hdac-1 is ubiquitous in early embryos (2-16 hr postfertilization), but at later stages (36 and 48 hr postfertilization), it is primarily restricted to the branchial arches, fin bud mesenchyme, and hindbrain. We report the phenotypes of hdac-1 homozygous mutant embryos and embryos injected with an hdac-1 antisense morpholino. These embryos possess a complex phenotype affecting several embryonic structures. We observed developmental abnormalities in the heart and neural epithelial structures, including the retina and the loss of craniofacial cartilage and pectoral fins. Developmental Dynamics, 2004. Copyright 2004 Wiley-Liss, Inc.