PUBLICATION
Histone deacetylase 1 (HDAC-1) required for the normal formation of craniofacial cartilage and pectoral fins of the zebrafish
- Authors
- Pillai, R., Coverdale, L.E., Dubey, G., and Martin, C.C.
- ID
- ZDB-PUB-040920-6
- Date
- 2004
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 231(3): 647-654 (Journal)
- Registered Authors
- Martin, C. Cristofre
- Keywords
- histone deacetylase, cartilage, fin bud, heart, morpholino, insertional mutant, zebrafish
- MeSH Terms
-
- Embryo, Mammalian/drug effects
- Oligonucleotides, Antisense/pharmacology
- Histone Deacetylases/metabolism
- Histone Deacetylases/physiology*
- Alcian Blue/metabolism
- Animals
- Zebrafish Proteins/metabolism
- Zebrafish Proteins/physiology*
- Embryo, Nonmammalian
- Mutagenesis, Insertional
- Skull/embryology*
- Embryonic Development
- Homozygote
- Cartilage/embryology*
- Time Factors
- In Situ Hybridization
- Histone Deacetylase 1
- Extremities/embryology*
- Zebrafish/embryology*
- Gene Expression Regulation, Developmental
- PubMed
- 15376317 Full text @ Dev. Dyn.
Citation
Pillai, R., Coverdale, L.E., Dubey, G., and Martin, C.C. (2004) Histone deacetylase 1 (HDAC-1) required for the normal formation of craniofacial cartilage and pectoral fins of the zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 231(3):647-654.
Abstract
Histone deacetylases interact with nucleosomes to facilitate the formation of transcriptionally repressed chromatin. In the present study, we show that histone deacetylase 1 (hdac-1) is expressed throughout embryonic development of the zebrafish. The expression of hdac-1 is ubiquitous in early embryos (2-16 hr postfertilization), but at later stages (36 and 48 hr postfertilization), it is primarily restricted to the branchial arches, fin bud mesenchyme, and hindbrain. We report the phenotypes of hdac-1 homozygous mutant embryos and embryos injected with an hdac-1 antisense morpholino. These embryos possess a complex phenotype affecting several embryonic structures. We observed developmental abnormalities in the heart and neural epithelial structures, including the retina and the loss of craniofacial cartilage and pectoral fins. Developmental Dynamics, 2004. Copyright 2004 Wiley-Liss, Inc.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping