PUBLICATION
Essential role of BCL9-2 in the switch between β-catenin's adhesive and transcriptional functions
- Authors
- Brembeck, F.H., Schwarz-Romond, T., Bakkers, J., Wilhelm, S., Hammerschmidt, M., and Birchmeier, W.
- ID
- ZDB-PUB-040920-3
- Date
- 2004
- Source
- Genes & Development 18(18): 2225-2230 (Journal)
- Registered Authors
- Bakkers, Jeroen, Hammerschmidt, Matthias
- Keywords
- BCL9/legless; beta-catenin; Wnt8; tyrosine phosphorylation; epithelial–mesenchymal transition; mesoderm patterning
- MeSH Terms
-
- Animals
- Body Patterning
- Carrier Proteins/physiology*
- Cell Adhesion/physiology
- Cell Line, Transformed
- Cell Membrane/metabolism
- Cell Movement
- Cell Nucleus/metabolism
- Cells, Cultured
- Cytoskeletal Proteins/genetics
- Cytoskeletal Proteins/metabolism*
- Embryo, Nonmammalian
- Epithelial Cells/physiology
- Humans
- Mesoderm/cytology
- Mesoderm/physiology
- Mice
- Neoplasm Proteins/physiology*
- Phosphorylation
- Protein Transport/physiology
- Proteins/metabolism
- RNA Interference
- Sequence Homology, Amino Acid
- Trans-Activators/genetics
- Trans-Activators/metabolism*
- Transcription, Genetic*
- Tyrosine/metabolism
- Wnt Proteins
- Zebrafish/embryology
- Zebrafish Proteins
- beta Catenin
- PubMed
- 15371335 Full text @ Genes & Dev.
Citation
Brembeck, F.H., Schwarz-Romond, T., Bakkers, J., Wilhelm, S., Hammerschmidt, M., and Birchmeier, W. (2004) Essential role of BCL9-2 in the switch between β-catenin's adhesive and transcriptional functions. Genes & Development. 18(18):2225-2230.
Abstract
beta-Catenin controls both cadherin-mediated cell adhesion and activation of Wnt target genes. We demonstrate here that the beta-catenin-binding protein BCL9-2, a homolog of the human proto-oncogene product BCL9, induces epithelial-mesenchymal transitions of nontransformed cells and increases beta-catenin-dependent transcription. RNA interference of BCL9-2 in carcinoma cells induces an epithelial phenotype and translocates beta-catenin from the nucleus to the cell membrane. The switch between beta-catenin's adhesive and transcriptional functions is modulated by phosphorylation of Tyr 142 of beta-catenin, which favors BCL9-2 binding and precludes interaction with alpha-catenin. During zebrafish embryogenesis, BCL9-2 acts in the Wnt8-signaling pathway and regulates mesoderm patterning.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping