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ZFIN ID: ZDB-PUB-040826-3
Sequence, linkage mapping and early developmental expression of the intestinal-type fatty acid-binding protein gene (fabp2) from zebrafish (Danio rerio)
Sharma, M.K., Denovan-Wright, E.M., Degrave, A., Thisse, C., Thisse, B., and Wright, J.M.
Date: 2004
Source: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 138(4): 391-398 (Journal)
Registered Authors: Degrave, Agnes, Sharma, Mukesh, Thisse, Bernard, Thisse, Christine, Wright, Jonathan M.
Keywords: Fabp2; Gene structure; Radiation hybrid-mapping; Transcription start site; RT-PCR; Whole-mount in situ hybridization; Pufferfish; Yolk syncytial layer
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carrier Proteins/biosynthesis*
  • Carrier Proteins/genetics*
  • Cloning, Molecular
  • Exons
  • Fatty Acid-Binding Proteins
  • Fatty Acids/chemistry
  • Genetic Linkage*
  • Introns
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • RNA/chemistry
  • RNA, Messenger/metabolism
  • Radiation Hybrid Mapping
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Time Factors
  • Tissue Distribution
  • Transcription, Genetic
  • Zebrafish
  • Zebrafish Proteins/biosynthesis*
  • Zebrafish Proteins/genetics*
PubMed: 15325340 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
ABSTRACT
The intestinal fatty acid-binding protein (I-FABP) shows binding specificity for long-chain fatty acids and is proposed to be involved in uptake of dietary fatty acids and their intracellular transport. We have determined the sequence of the gene encoding I-FABP in zebrafish. The zebrafish I-FABP gene contains four exons interrupted by three introns. Radiation hybrid mapping assigned the I-FABP gene to linkage group 1. A 924 bp sequence 5' upstream of the initiation codon in the I-FABP gene contained several putative cis-acting regulatory elements. In adult zebrafish, reverse transcription-polymerase chain reaction (RT-PCR) detected I-FABP mRNA in intestine, brain, liver, muscle and testis. Quantitative RT-PCR demonstrated that I-FABP mRNA was most abundant in intestine, followed by brain. I-FABP mRNA levels were very low in muscle, testis, heart, liver, skin and ovary. RT-PCR using total RNA extracted from zebrafish embryos detected I-FABP mRNA as early as 12 h post-fertilization. Whole-mount in situ hybridization to zebrafish embryos detected I-FABP mRNA in the yolk syncytial layer (YSL) at early somitogenesis. Later during embryonic development the I-FABP mRNA was detected in the intestinal bulb, liver and pancreas primordium. Expression in YSL, liver or pancreas has not been previously reported for fish or mammalian I-FABP genes and may be related to specific physiological differences between fishes and mammals.
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