PUBLICATION
Sequence, linkage mapping and early developmental expression of the intestinal-type fatty acid-binding protein gene (fabp2) from zebrafish (Danio rerio)
- Authors
- Sharma, M.K., Denovan-Wright, E.M., Degrave, A., Thisse, C., Thisse, B., and Wright, J.M.
- ID
- ZDB-PUB-040826-3
- Date
- 2004
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 138(4): 391-398 (Journal)
- Registered Authors
- Degrave, Agnes, Sharma, Mukesh, Thisse, Bernard, Thisse, Christine, Wright, Jonathan M.
- Keywords
- Fabp2; Gene structure; Radiation hybrid-mapping; Transcription start site; RT-PCR; Whole-mount in situ hybridization; Pufferfish; Yolk syncytial layer
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Carrier Proteins/biosynthesis*
- Carrier Proteins/genetics*
- Cloning, Molecular
- Exons
- Fatty Acid-Binding Proteins
- Fatty Acids/chemistry
- Genetic Linkage*
- Introns
- Molecular Sequence Data
- Promoter Regions, Genetic
- RNA/chemistry
- RNA, Messenger/metabolism
- Radiation Hybrid Mapping
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Time Factors
- Tissue Distribution
- Transcription, Genetic
- Zebrafish
- Zebrafish Proteins/biosynthesis*
- Zebrafish Proteins/genetics*
- PubMed
- 15325340 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Sharma, M.K., Denovan-Wright, E.M., Degrave, A., Thisse, C., Thisse, B., and Wright, J.M. (2004) Sequence, linkage mapping and early developmental expression of the intestinal-type fatty acid-binding protein gene (fabp2) from zebrafish (Danio rerio). Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 138(4):391-398.
Abstract
The intestinal fatty acid-binding protein (I-FABP) shows binding specificity for long-chain fatty acids and is proposed to be involved in uptake of dietary fatty acids and their intracellular transport. We have determined the sequence of the gene encoding I-FABP in zebrafish. The zebrafish I-FABP gene contains four exons interrupted by three introns. Radiation hybrid mapping assigned the I-FABP gene to linkage group 1. A 924 bp sequence 5' upstream of the initiation codon in the I-FABP gene contained several putative cis-acting regulatory elements. In adult zebrafish, reverse transcription-polymerase chain reaction (RT-PCR) detected I-FABP mRNA in intestine, brain, liver, muscle and testis. Quantitative RT-PCR demonstrated that I-FABP mRNA was most abundant in intestine, followed by brain. I-FABP mRNA levels were very low in muscle, testis, heart, liver, skin and ovary. RT-PCR using total RNA extracted from zebrafish embryos detected I-FABP mRNA as early as 12 h post-fertilization. Whole-mount in situ hybridization to zebrafish embryos detected I-FABP mRNA in the yolk syncytial layer (YSL) at early somitogenesis. Later during embryonic development the I-FABP mRNA was detected in the intestinal bulb, liver and pancreas primordium. Expression in YSL, liver or pancreas has not been previously reported for fish or mammalian I-FABP genes and may be related to specific physiological differences between fishes and mammals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping