PUBLICATION
receptor tyrosine phosphatase ? is required for Delta/Notch signalling and cyclic gene expression in the presomitic mesoderm
- Authors
- Aerne, B., and Ish-Horowicz, D.
- ID
- ZDB-PUB-040701-3
- Date
- 2004
- Source
- Development (Cambridge, England) 131(14): 3391-3399 (Journal)
- Registered Authors
- Aerne, Birgit
- Keywords
- none
- MeSH Terms
-
- Base Sequence
- Animals
- Intracellular Signaling Peptides and Proteins
- Gene Expression Regulation, Developmental*
- Receptors, Notch
- Gene Library
- Protein Biosynthesis
- Protein Structure, Tertiary
- Molecular Sequence Data
- Transcription, Genetic
- Receptor-Like Protein Tyrosine Phosphatases, Class 2
- Oscillometry
- Zebrafish
- Signal Transduction*
- Chick Embryo
- Cloning, Molecular
- Membrane Proteins/metabolism*
- Oligonucleotides, Antisense/chemistry
- In Situ Hybridization
- Protein Tyrosine Phosphatases/metabolism
- Protein Tyrosine Phosphatases/physiology*
- Mesoderm/metabolism*
- PubMed
- 15226256 Full text @ Development
Citation
Aerne, B., and Ish-Horowicz, D. (2004) receptor tyrosine phosphatase ? is required for Delta/Notch signalling and cyclic gene expression in the presomitic mesoderm. Development (Cambridge, England). 131(14):3391-3399.
Abstract
Segmentation in vertebrate embryos is controlled by a biochemical oscillator ('segmentation clock') intrinsic to the cells in the unsegmented presomitic mesoderm, and is manifested in cyclic transcription of genes involved in establishing somite polarity and boundaries. We show that the receptor protein tyrosine phosphatase psi (RPTPpsi) gene is essential for normal functioning of the somitogenesis clock in zebrafish. We show that reduction of RPTPpsi activity using morpholino antisense oligonucleotides results in severe disruption of the segmental pattern of the embryo, and loss of cyclic gene expression in the presomitic mesoderm. Analysis of cyclic genes in RPTPpsi morphant embryos indicates an important requirement for RPTPpsi in the control of the somitogenesis clock upstream of or in parallel with Delta/Notch signalling. Impairing RPTPpsi activity also interferes with convergent extension during gastrulation. We discuss this dual requirement for RPTPpsi in terms of potential functions in Notch and Wnt signalling.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping