PUBLICATION

receptor tyrosine phosphatase ? is required for Delta/Notch signalling and cyclic gene expression in the presomitic mesoderm

Authors
Aerne, B., and Ish-Horowicz, D.
ID
ZDB-PUB-040701-3
Date
2004
Source
Development (Cambridge, England)   131(14): 3391-3399 (Journal)
Registered Authors
Aerne, Birgit
Keywords
none
MeSH Terms
  • Transcription, Genetic
  • Receptors, Notch
  • Cloning, Molecular
  • Zebrafish
  • Molecular Sequence Data
  • Base Sequence
  • Gene Library
  • Intracellular Signaling Peptides and Proteins
  • Animals
  • Oscillometry
  • Protein Tyrosine Phosphatases/metabolism
  • Protein Tyrosine Phosphatases/physiology*
  • Protein Structure, Tertiary
  • Receptor-Like Protein Tyrosine Phosphatases, Class 2
  • Mesoderm/metabolism*
  • In Situ Hybridization
  • Chick Embryo
  • Membrane Proteins/metabolism*
  • Gene Expression Regulation, Developmental*
  • Oligonucleotides, Antisense/chemistry
  • Signal Transduction*
  • Protein Biosynthesis
(all 22)
PubMed
15226256 Full text @ Development
Abstract
Segmentation in vertebrate embryos is controlled by a biochemical oscillator ('segmentation clock') intrinsic to the cells in the unsegmented presomitic mesoderm, and is manifested in cyclic transcription of genes involved in establishing somite polarity and boundaries. We show that the receptor protein tyrosine phosphatase psi (RPTPpsi) gene is essential for normal functioning of the somitogenesis clock in zebrafish. We show that reduction of RPTPpsi activity using morpholino antisense oligonucleotides results in severe disruption of the segmental pattern of the embryo, and loss of cyclic gene expression in the presomitic mesoderm. Analysis of cyclic genes in RPTPpsi morphant embryos indicates an important requirement for RPTPpsi in the control of the somitogenesis clock upstream of or in parallel with Delta/Notch signalling. Impairing RPTPpsi activity also interferes with convergent extension during gastrulation. We discuss this dual requirement for RPTPpsi in terms of potential functions in Notch and Wnt signalling.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Aerne, B., and Ish-Horowicz, D. (2004) receptor tyrosine phosphatase ? is required for Delta/Notch signalling and cyclic gene expression in the presomitic mesoderm. Development (Cambridge, England). 131(14):3391-3399.