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ZFIN ID: ZDB-PUB-040510-6
Cadherin-2 and cadherin-4 in developing, adult and regenerating zebrafish cerebellum
Liu, Q., Azodi, E., Kerstetter, A.E., and Wilson, A.L.
Date: 2004
Source: Brain research. Developmental brain research   150(1): 63-71 (Journal)
Registered Authors: Liu, Qin
Keywords: none
MeSH Terms:
  • Animals
  • Cadherins/genetics
  • Cadherins/metabolism*
  • Cerebellum/cytology
  • Cerebellum/growth & development*
  • Cerebellum/metabolism*
  • Down-Regulation/genetics
  • Larva/cytology
  • Larva/growth & development*
  • Larva/metabolism*
  • Nerve Regeneration/genetics
  • Oligonucleotides, Antisense/pharmacology
  • Purkinje Cells/cytology
  • Purkinje Cells/metabolism
  • RNA, Messenger/metabolism
  • Up-Regulation/genetics
  • Zebrafish/anatomy & histology
  • Zebrafish/growth & development*
  • Zebrafish/metabolism*
PubMed: 15126039 Full text @ Brain Res. Dev. Brain Res.
Cadherins are cell adhesion molecules that regulate development of a variety of tissues and maintenance of adult structures. In this study, we examined expression of two zebrafish classical cadherins, cadherin-2 and cadherin-4, in the cerebellum of developing, normal adult, and regenerating adult zebrafish using in situ hybridization and immunohistochemical methods. Cadherin-2 was widely expressed by the cerebellum of embryonic (24-50-h post fertilization) and larval zebrafish (3-14 days). Cadherin-2 expression became much reduced in the adult cerebellum, but it was greatly up-regulated in the regenerating adult cerebellum. Cadherin-4 was not detected in the embryonic cerebellum, but it was expressed in the Purkinje cells of the larval and adult cerebellum. To gain insight into cadherin-2 role in the formation of the cerebellum, we analyzed embryos injected with a specific cadherin-2 antisense morpholino oligonucleotide (cdh2MO1), and found that the cerebellar development of the cdh2MO1-injected embryos was severely disrupted. This phenotype was confirmed by examining a cadherin-2 mutant, glass onion. Our results suggest that cadherins are crucial for the normal development of the zebrafish cerebellum, and they may also be involved in the regeneration of injured fish cerebellum.