PUBLICATION
Cloning and expression analysis of zebrafish tob1 gene
- Authors
- Shi, K., Zhang, L., and Meng, A.
- ID
- ZDB-PUB-040421-1
- Date
- 2004
- Source
- Development genes and evolution 214(6): 309-311 (Journal)
- Registered Authors
- Meng, Anming, Shi, Kehui, Zhang, Lixia
- Keywords
- Btg/Tob family, tob1, Zebrafish, Embryo, Somites
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cloning, Molecular*
- Conserved Sequence
- Gene Expression Profiling
- In Situ Hybridization
- Molecular Sequence Data
- Sequence Alignment
- Sequence Homology, Amino Acid
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 15098123 Full text @ Dev. Genes Evol.
Citation
Shi, K., Zhang, L., and Meng, A. (2004) Cloning and expression analysis of zebrafish tob1 gene. Development genes and evolution. 214(6):309-311.
Abstract
Many members of the Btg/Tob family, which possess anti-proliferation activity, have been identified in mammals but their physiological functions are poorly understood. We have identified a zebrafish tob1 gene, which encodes a peptide of 322 amino acids. The expression pattern of zebrafish tob1 during early embryogenesis is examined by RT-PCR and whole-mount in situ hybridization. The maternal tob1 transcript is abundant in fertilized eggs and blastulas. The expression of tob1 ceases during midgastrulation and early segmentation, when it is expressed and restricted to the notochord and somites. On day 2, expression occurs in the pectoral fin bud but not in other tissues. It will be interesting to investigate whether Tob1 is required for controlling cell proliferation during vertebrate embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping