PUBLICATION
A Medaka Gene Map: The Trace of Ancestral Vertebrate Proto-Chromosomes Revealed by Comparative Gene Mapping
- Authors
- Naruse, K., Tanaka, M., Mita, K., Shima, A., Postlethwait, J., and Mitani, H.
- ID
- ZDB-PUB-040415-7
- Date
- 2004
- Source
- Genome research 14(5): 820-828 (Journal)
- Registered Authors
- Naruse, Kiyoshi, Postlethwait, John H., Shima, Akihiro, Tanaka, Minoru
- Keywords
- none
- MeSH Terms
-
- Animals
- Humans
- Chromosome Mapping/methods*
- Genetic Linkage/genetics
- Genome, Human
- Genome
- Gene Duplication
- Multigene Family/genetics
- Gene Silencing/physiology
- Gene Expression Regulation/genetics
- Oryzias/genetics*
- Skates, Fish/genetics
- Zebrafish/genetics
- Conserved Sequence/genetics
- Expressed Sequence Tags
- Chromosomes/genetics*
- Chromosomes, Human/genetics
- Ploidies
- Synteny/genetics
- Evolution, Molecular*
- PubMed
- 15078856 Full text @ Genome Res.
Citation
Naruse, K., Tanaka, M., Mita, K., Shima, A., Postlethwait, J., and Mitani, H. (2004) A Medaka Gene Map: The Trace of Ancestral Vertebrate Proto-Chromosomes Revealed by Comparative Gene Mapping. Genome research. 14(5):820-828.
Abstract
The mapping of Hox clusters and many duplicated genes in zebrafish indicated an extra whole-genome duplication in ray-fined fish. However, to reconstruct the preduplication chromosomes (proto-chromosomes), the comparative genomic studies of more distantly related teleosts are essential. Medaka and zebrafish are ideal for this purpose, because their lineages separated from their last common ancestor approximately 140 million years ago. To reconstruct ancient vertebrate chromosomes, including the chromosomes of the vertebrate ancestor of humans from 450 million years ago, we mapped 818 genes and expressed sequence tags (ESTs) on a single meiotic backcross panel obtained from inbred strains of the medaka, Oryzias latipes. Comparisons of linkage relationships of orthologous genes among three species of vertebrates (medaka, zebrafish, and human) indicate the number and content of the chromosomes of the last common ancestor of ray-fined fish and lobe-fined fish (including humans), and the extra whole genome duplication event in the ray-fin lineage occurred in the common ancestor of perhaps all teleosts.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping