PUBLICATION
Visual pigment phosphorylation but not transducin translocation can contribute to light adaptation in zebrafish cones
- Authors
- Kennedy, M.J., Dunn, F.A., and Hurley, J.B.
- ID
- ZDB-PUB-040405-5
- Date
- 2004
- Source
- Neuron 41(6): 915-928 (Journal)
- Registered Authors
- Hurley, James B.
- Keywords
- none
- MeSH Terms
-
- Animals
- Retinal Cone Photoreceptor Cells/cytology
- Retinal Cone Photoreceptor Cells/drug effects
- Retinal Cone Photoreceptor Cells/metabolism*
- Cytoplasm/drug effects
- Cytoplasm/metabolism
- Retinal Pigments/genetics
- Retinal Pigments/metabolism*
- Organ Culture Techniques
- Vision, Ocular/physiology*
- Vision, Ocular/radiation effects
- Calcium/metabolism
- Calcium/pharmacology
- Phosphorylation/drug effects
- Phosphorylation/radiation effects
- Transducin/metabolism*
- Rod Opsins/genetics
- Rod Opsins/metabolism
- Mutation/genetics
- Photic Stimulation
- Light
- Calcium Signaling/drug effects
- Calcium Signaling/physiology
- Immunohistochemistry
- Zebrafish
- Adaptation, Ocular/drug effects
- Adaptation, Ocular/physiology*
- Protein Transport/physiology
- Protein Transport/radiation effects
- PubMed
- 15046724 Full text @ Neuron
Citation
Kennedy, M.J., Dunn, F.A., and Hurley, J.B. (2004) Visual pigment phosphorylation but not transducin translocation can contribute to light adaptation in zebrafish cones. Neuron. 41(6):915-928.
Abstract
The ability of cone photoreceptors to adapt to light is extraordinary. In this study we evaluated two biochemical processes, visual pigment phosphorylation and transducin translocation, for their ability to contribute to light adaptation in zebrafish cones. Since cytoplasmic Ca(2+) regulates light adaptation, the sensitivities of these processes to both light and Ca(2+) were examined. Cytoplasmic Ca(2+) regulates the sites of light-stimulated phosphorylation. Unexpectedly, we found that Ca(2+) also regulates the extent of phosphorylation of unbleached cone pigments. Immunocytochemical analyses revealed that neither light nor cytoplasmic Ca(2+) influences the localization of transducin in zebrafish cones.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping