PUBLICATION
Three modules of zebrafish Mind bomb work cooperatively to promote Delta ubiquitination and endocytosis
- Authors
- Chen, W., and Casey Corliss, D.
- ID
- ZDB-PUB-040312-3
- Date
- 2004
- Source
- Developmental Biology 267(2): 361-373 (Journal)
- Registered Authors
- Chen, Wenbiao
- Keywords
- Delta, Notch, Neurogenesis, E3 ubiquitin ligase, Endocytosis, Lateral inhibition
- MeSH Terms
-
- Fluorescent Antibody Technique
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Ubiquitins/metabolism*
- Signal Transduction/physiology
- Amino Acid Sequence
- Precipitin Tests
- Membrane Proteins/metabolism*
- Zebrafish/embryology*
- Immunoblotting
- DNA, Complementary/genetics
- Intracellular Signaling Peptides and Proteins
- In Situ Hybridization
- Ubiquitin-Protein Ligases/genetics
- Ubiquitin-Protein Ligases/metabolism*
- Molecular Sequence Data
- Endocytosis*
- Animals
- Immunohistochemistry
- DNA Primers
- Sequence Alignment
- Plasmids
- Receptors, Notch
- PubMed
- 15013799 Full text @ Dev. Biol.
Citation
Chen, W., and Casey Corliss, D. (2004) Three modules of zebrafish Mind bomb work cooperatively to promote Delta ubiquitination and endocytosis. Developmental Biology. 267(2):361-373.
Abstract
Precise regulation of Notch signaling activity is critical for development of many different tissues. Here, we show that the zebrafish insertional mutation Hi904 attenuates Notch signaling, and is allelic to mind bomb. We show that Mind bomb protein displays E3 ubiquitin ligase activity in vitro and that it is associated with Delta and enhances its ubiquitination and internalization in transfected cells. Furthermore, by functional analysis of three conserved regions of Mind bomb, we show that the N-terminal half is required for Delta association, the ankyrin repeats are important for Delta internalization, and the ring fingers are required for Delta ubiquitination. Thus, the three functionally distinct modules of Mind bomb work cooperatively to regulate Notch signaling by associating with, ubiquitinating, and internalizing Delta
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping