|ZFIN ID: ZDB-PUB-040304-2|
Zebrafish serotonin-N-acetyltransferase-2 gene regulation: Pineal-restrictive downstream module (PRDM) contains a functional E-box and three photoreceptor conserved elements
Appelbaum, L., Toyama, R., Dawid, I.B., Klein, D.C., Baler, R., and Gothilf, Y.
|Source:||Molecular endocrinology (Baltimore, Md.) 18(5): 1210-1221 (Journal)|
|Registered Authors:||Dawid, Igor B., Gothilf, Yoav, Klein, David C., Toyama, Reiko|
|PubMed:||14988431 Full text @ Mol. Endocrinol.|
Appelbaum, L., Toyama, R., Dawid, I.B., Klein, D.C., Baler, R., and Gothilf, Y. (2004) Zebrafish serotonin-N-acetyltransferase-2 gene regulation: Pineal-restrictive downstream module (PRDM) contains a functional E-box and three photoreceptor conserved elements. Molecular endocrinology (Baltimore, Md.). 18(5):1210-1221.
ABSTRACTPineal function is defined by a set of very narrowly expressed genes that encode proteins required for photoperiodic transduction and rhythmic melatonin secretion. One of these proteins is serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT), which controls the daily rhythm in melatonin production. Here, pineal-specific expression of the zebrafish aanat-2 (zfaanat-2) was studied using in vivo transient expression analyses of promoter-reporter constructs; this revealed that specificity is determined by two regions located 12 kb away from each other. One is the 5'-flanking region and the other is a 257 bp sequence, located 6 kb downstream of the transcribed region. This 3' sequence, designated pineal-restrictive downstream module (PRDM), has a dual function: enhancement of pineal expression and inhibition of extra-pineal expression. The former is an autonomic property of PRDM while the later function requires interaction with the upstream regulatory region of zfaanat-2. Functional analyses of the PRDM sequence revealed that three photoreceptor conserved elements (PCE; TAATC) and a single perfect E-box (CACGTG) are crucial for the dual function of PRDM. These results indicate that pineal-specificity of zfaanat-2 is determined by the dual functionality of the PRDM and the interaction between upstream regulatory region and downstream PCEs and E-box element.
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