PUBLICATION
DRAGON: a member of the repulsive guidance molecule-related family of neuronal- and muscle-expressed membrane proteins is regulated by DRG11 and has neuronal adhesive properties
- Authors
- Samad, T.A., Srinivasan, A., Karchewski, L.A., Jeong, S.J., Campagna, J.A., Ji, R.R., Fabrizio, D.A., Zhang, Y., Lin, H.Y., Bell, E., and Woolf, C.J.
- ID
- ZDB-PUB-040304-11
- Date
- 2004
- Source
- The Journal of neuroscience : the official journal of the Society for Neuroscience 24(8): 2027-2036 (Journal)
- Registered Authors
- Srinivasan, Ashok
- Keywords
- development, GPI anchor, neuron, spinal cord, brain, muscle
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Brain/embryology
- Brain/metabolism
- Cell Line
- Cloning, Molecular
- Conserved Sequence/genetics
- GPI-Linked Proteins
- Ganglia, Spinal/embryology
- Ganglia, Spinal/metabolism
- Gene Expression Regulation, Developmental
- Glycosylphosphatidylinositols/metabolism
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism*
- Humans
- Membrane Proteins/genetics
- Membrane Proteins/metabolism*
- Mice
- Molecular Sequence Data
- Multigene Family/genetics
- Muscle Proteins/genetics
- Muscle Proteins/metabolism
- Nerve Tissue Proteins/biosynthesis
- Nerve Tissue Proteins/genetics*
- Nerve Tissue Proteins/metabolism*
- Neural Cell Adhesion Molecules/genetics
- Neural Cell Adhesion Molecules/metabolism*
- Neurons/cytology
- Neurons/metabolism
- Organ Specificity
- RNA, Messenger/metabolism
- Sequence Homology, Amino Acid
- Spinal Cord/embryology
- Spinal Cord/metabolism
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Zebrafish
- PubMed
- 14985445 Full text @ J. Neurosci.
Citation
Samad, T.A., Srinivasan, A., Karchewski, L.A., Jeong, S.J., Campagna, J.A., Ji, R.R., Fabrizio, D.A., Zhang, Y., Lin, H.Y., Bell, E., and Woolf, C.J. (2004) DRAGON: a member of the repulsive guidance molecule-related family of neuronal- and muscle-expressed membrane proteins is regulated by DRG11 and has neuronal adhesive properties. The Journal of neuroscience : the official journal of the Society for Neuroscience. 24(8):2027-2036.
Abstract
DRG11, a transcription factor expressed in embryonic dorsal root ganglion (DRG) and dorsal horn neurons, has a role in the development of sensory circuits. We have used a genomic binding strategy to screen for the promoter region of genes regulated by DRG11. One gene with a promoter region binding to the DNA binding domain of DRG11 encodes a novel membrane-associated [glycosyl-phosphatidylinositol (GPI)-anchored] protein that we call DRAGON. DRAGON expression is transcriptionally regulated by DRG11, and it is coexpressed with DRG11 in embryonic DRG and spinal cord. DRAGON expression in these areas is reduced in DRG11 null mutants. DRAGON is expressed, however, in the neural tube before DRG11, and unlike DRG11 it is expressed in the brain and therefore must be regulated by other transcriptional regulatory elements. DRAGON shares high sequence homology with two other GPI-anchored membrane proteins: the mouse ortholog of chick repulsive guidance molecule (mRGM), which is expressed in the mouse nervous system in areas complementary to DRAGON, and DRAGON-like muscle (DL-M), the expression of which is restricted to skeletal and cardiac muscle. A comparative genomic analysis indicates that the family of RGM-related genes—mRGM, DRAGON, and DL-M—are highly conserved among mammals, zebrafish, chick, and Caenorhabditis elegans but not Drosophila. DRAGON, RGM, and DL-M mRNA expression in the zebrafish embryo is similar to that in the mouse. Neuronal cell adhesion assays indicate that DRAGON promotes and mRGM reduces adhesion of mouse DRG neurons. We show that DRAGON interacts with itself homophilically. The dynamic expression, ordered spatial localization, and adhesive properties of the RGM-related family of membrane-associated proteins are compatible with specific roles in development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping